Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/88678
Título: | Influence of ultrasound stimulation on the viability, proliferation and protein expression of osteoblasts and periodontal ligament fibroblasts |
Autor(es): | Pascoal, Selma Oliveira, Sofia Monteiro, Francisca Padrão, Jorge Costa, Rita Zille, Andrea Catarino, Susana Oliveira Silva, Filipe S. Pinho, Teresa Carvalho, Óscar Samuel Novais |
Palavras-chave: | Fibroblasts Orthodontic tooth movement Osteoblasts Ultrasound |
Data: | 3-Fev-2024 |
Editora: | Multidisciplinary Digital Publishing Institute (MDPI) |
Revista: | Biomedicines |
Citação: | Pascoal, S.; Oliveira, S.; Monteiro, F.; Padrão, J.; Costa, R.; Zille, A.; Catarino, S.O.; Silva, F.S.; Pinho, T.; Carvalho, Ó. Influence of Ultrasound Stimulation on the Viability, Proliferation and Protein Expression of Osteoblasts and Periodontal Ligament Fibroblasts. Biomedicines 2024, 12, 361. https://doi.org/10.3390/biomedicines12020361 |
Resumo(s): | Among the adjunctive procedures to accelerate orthodontic tooth movement (OTM), ultrasound (US) is a nonsurgical form of mechanical stimulus that has been explored as an alternative to the currently available treatments. This study aimed to clarify the role of US in OTM by exploring different stimulation parameters and their effects on the biological responses of cells involved in OTM. Human fetal osteoblasts and periodontal ligament fibroblasts cell lines were stimulated with US at 1.0 and 1.5 MHz central frequencies and power densities of 30 and 60 mW/cm2 in continuous mode for 5 and 10 min. Cellular proliferation, metabolic activity and protein expression were analyzed. The US parameters that significantly improved the metabolic activity were 1.0 MHz at 30 mW/cm2 for 5 min and 1.0 MHz at 60 mW/cm2 for 5 and 10 min for osteoblasts; and 1.0 MHz at 30 mW/cm2 for 5 min and 1.5 MHz at 60 mW/cm2 for 5 and 10 min for fibroblasts. By stimulating with these parameters, the expression of alkaline phosphatase was maintained, while osteoprotegerin synthesis was induced after three days of US stimulation. The US stimulation improved the biological activity of both osteoblasts and periodontal ligament fibroblasts, inducing their osteogenic differentiation. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/88678 |
DOI: | 10.3390/biomedicines12020361 |
e-ISSN: | 2227-9059 |
Versão da editora: | https://www.mdpi.com/2227-9059/12/2/361 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CMEMS - Artigos em revistas internacionais/Papers in international journals DET/2C2T - Artigos em revistas internacionais com arbitragem científica |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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biomedicines-12-00361.pdf | 1,61 MB | Adobe PDF | Ver/Abrir |
Este trabalho está licenciado sob uma Licença Creative Commons