Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/88678
Registo completo
Campo DC | Valor | Idioma |
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dc.contributor.author | Pascoal, Selma | por |
dc.contributor.author | Oliveira, Sofia | por |
dc.contributor.author | Monteiro, Francisca | por |
dc.contributor.author | Padrão, Jorge | por |
dc.contributor.author | Costa, Rita | por |
dc.contributor.author | Zille, Andrea | por |
dc.contributor.author | Catarino, Susana Oliveira | por |
dc.contributor.author | Silva, Filipe S. | por |
dc.contributor.author | Pinho, Teresa | por |
dc.contributor.author | Carvalho, Óscar Samuel Novais | por |
dc.date.accessioned | 2024-02-08T09:39:23Z | - |
dc.date.available | 2024-02-08T09:39:23Z | - |
dc.date.issued | 2024-02-03 | - |
dc.identifier.citation | Pascoal, S.; Oliveira, S.; Monteiro, F.; Padrão, J.; Costa, R.; Zille, A.; Catarino, S.O.; Silva, F.S.; Pinho, T.; Carvalho, Ó. Influence of Ultrasound Stimulation on the Viability, Proliferation and Protein Expression of Osteoblasts and Periodontal Ligament Fibroblasts. Biomedicines 2024, 12, 361. https://doi.org/10.3390/biomedicines12020361 | por |
dc.identifier.uri | https://hdl.handle.net/1822/88678 | - |
dc.description.abstract | Among the adjunctive procedures to accelerate orthodontic tooth movement (OTM), ultrasound (US) is a nonsurgical form of mechanical stimulus that has been explored as an alternative to the currently available treatments. This study aimed to clarify the role of US in OTM by exploring different stimulation parameters and their effects on the biological responses of cells involved in OTM. Human fetal osteoblasts and periodontal ligament fibroblasts cell lines were stimulated with US at 1.0 and 1.5 MHz central frequencies and power densities of 30 and 60 mW/cm2 in continuous mode for 5 and 10 min. Cellular proliferation, metabolic activity and protein expression were analyzed. The US parameters that significantly improved the metabolic activity were 1.0 MHz at 30 mW/cm2 for 5 min and 1.0 MHz at 60 mW/cm2 for 5 and 10 min for osteoblasts; and 1.0 MHz at 30 mW/cm2 for 5 min and 1.5 MHz at 60 mW/cm2 for 5 and 10 min for fibroblasts. By stimulating with these parameters, the expression of alkaline phosphatase was maintained, while osteoprotegerin synthesis was induced after three days of US stimulation. The US stimulation improved the biological activity of both osteoblasts and periodontal ligament fibroblasts, inducing their osteogenic differentiation. | por |
dc.description.sponsorship | This in vitro study was supported by FCT (Fundação para a Ciência e Tecnologia) in the scope of the grants UI/BD/150951/2021 and UI/BD/09375/2020, the funding contract 2020.00215.CEECIND (DOI: 10.54499/2020.00215.CEECIND/CP1600/CT0009) and through the projects UIDB/04436/2020, UIDP/04436/2020, FunFibRai—PTDC/EME-EME/4197/2021 (DOI: 10.54499/PTDC/EME-EME/4197/ 2021), BrainStimMap—PTDC/EME-EME/1681/2021 (DOI: 10.54499/PTDC/EME-EME/1681/2021), AlignAgen-GI2-CESPU-2022 and UID/CTM/00264/2021 project of 2C2T under the COMPETE and FCT/MCTES (PIDDAC), co-financed by FEDER through the PT2020 program. | por |
dc.language.iso | eng | por |
dc.publisher | Multidisciplinary Digital Publishing Institute (MDPI) | por |
dc.relation | info:eu-repo/grantAgreement/FCT/OE/UI%2FBD%2F150951%2F2021/PT | por |
dc.relation | UI/BD/09375/2020 | por |
dc.relation | info:eu-repo/grantAgreement/FCT/CEEC IND 3ed/2020.00215.CEECIND%2FCP1600%2FCT0009/PT | por |
dc.relation | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04436%2F2020/PT | por |
dc.relation | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04436%2F2020/PT | por |
dc.relation | info:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FEME-EME%2F4197%2F2021/PT | por |
dc.relation | info:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FEME-EME%2F1681%2F2021/PT | por |
dc.relation | AlignAgen-GI2-CESPU-2022 | por |
dc.relation | UID/CTM/00264/2021 | por |
dc.rights | openAccess | por |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | por |
dc.subject | Fibroblasts | por |
dc.subject | Orthodontic tooth movement | por |
dc.subject | Osteoblasts | por |
dc.subject | Ultrasound | por |
dc.title | Influence of ultrasound stimulation on the viability, proliferation and protein expression of osteoblasts and periodontal ligament fibroblasts | por |
dc.type | article | por |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://www.mdpi.com/2227-9059/12/2/361 | por |
oaire.citationStartPage | 1 | por |
oaire.citationEndPage | 13 | por |
oaire.citationIssue | 2 | por |
oaire.citationVolume | 12 | por |
dc.identifier.eissn | 2227-9059 | - |
dc.identifier.doi | 10.3390/biomedicines12020361 | por |
dc.subject.fos | Ciências Médicas::Biotecnologia Médica | por |
sdum.journal | Biomedicines | por |
oaire.version | VoR | por |
dc.identifier.articlenumber | 361 | por |
dc.subject.ods | Saúde de qualidade | por |
Aparece nas coleções: | CMEMS - Artigos em revistas internacionais/Papers in international journals DET/2C2T - Artigos em revistas internacionais com arbitragem científica |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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biomedicines-12-00361.pdf | 1,61 MB | Adobe PDF | Ver/Abrir |
Este trabalho está licenciado sob uma Licença Creative Commons