Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/60119

TitleBiofunctional nanofibrous substrate for local TNF-capturing as a strategy to control inflammation in arthritic joints
Author(s)Bacelo, Elisa
Alves da Silva, Marta
Cunha, Cristina
Faria, Susana
Carvalho, Agostinho
Reis, R. L.
Martins, Albino
Neves, Nuno M.
Keywordsantibody immobilization
electrospun nanofibers
TNF- capture
human articular chondrocytes
rheumatoid arthritis
TNF-α capture
Issue date8-Apr-2019
PublisherMultidisciplinary Digital Publishing Institute
JournalNanomaterials
Abstract(s)Rheumatoid arthritis (RA) is an autoimmune disease that affects the synovial cavity of joints, and its pathogenesis is associated with an increased expression of pro-inflammatory cytokines, namely tumour necrosis factor-alpha (TNF-α). It has been clinically shown to have an adequate response to systemic administration of TNF-α inhibitors, although with many shortcomings. To overcome such limitations, the immobilization of a TNF-α antibody on a nanofibrous substrate to promote a localized action is herein proposed. By using this approach, the antibody has its maximum therapeutic efficacy and a prolonged therapeutic benefit, avoiding the systemic side-effects associated with conventional biological agents’ therapies. To technically achieve such a purpose, the surface of electrospun nanofibers is initially activated and functionalized, allowing TNF-α antibody immobilization at a maximum concentration of 6 µg/mL. Experimental results evidence that the biofunctionalized nanofibrous substrate is effective in achieving a sustained capture of soluble TNF-α over time. Moreover, cell biology assays demonstrate that this system has no deleterious effect over human articular chondrocytes metabolism and activity. Therefore, the developed TNF-capturing system may represent a potential therapeutic approach for the local management of severely affected joints.
TypeArticle
URIhttp://hdl.handle.net/1822/60119
DOI10.3390/nano9040567
ISSN2079-4991
Peer-Reviewedyes
AccessOpen access
Appears in Collections:BUM - MDPI

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