Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/60119
Título: | Biofunctional nanofibrous substrate for local TNF-capturing as a strategy to control inflammation in arthritic joints |
Autor(es): | Bacelo, Elisa Alves da Silva, Marta Cunha, Cristina Faria, Susana Carvalho, Agostinho Reis, R. L. Martins, Albino Neves, Nuno M. |
Palavras-chave: | antibody immobilization electrospun nanofibers TNF- capture human articular chondrocytes rheumatoid arthritis TNF-α capture |
Data: | 8-Abr-2019 |
Editora: | Multidisciplinary Digital Publishing Institute |
Revista: | Nanomaterials |
Resumo(s): | Rheumatoid arthritis (RA) is an autoimmune disease that affects the synovial cavity of joints, and its pathogenesis is associated with an increased expression of pro-inflammatory cytokines, namely tumour necrosis factor-alpha (TNF-α). It has been clinically shown to have an adequate response to systemic administration of TNF-α inhibitors, although with many shortcomings. To overcome such limitations, the immobilization of a TNF-α antibody on a nanofibrous substrate to promote a localized action is herein proposed. By using this approach, the antibody has its maximum therapeutic efficacy and a prolonged therapeutic benefit, avoiding the systemic side-effects associated with conventional biological agents’ therapies. To technically achieve such a purpose, the surface of electrospun nanofibers is initially activated and functionalized, allowing TNF-α antibody immobilization at a maximum concentration of 6 µg/mL. Experimental results evidence that the biofunctionalized nanofibrous substrate is effective in achieving a sustained capture of soluble TNF-α over time. Moreover, cell biology assays demonstrate that this system has no deleterious effect over human articular chondrocytes metabolism and activity. Therefore, the developed TNF-capturing system may represent a potential therapeutic approach for the local management of severely affected joints. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/60119 |
DOI: | 10.3390/nano9040567 |
ISSN: | 2079-4991 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | ICVS - Artigos em revistas internacionais / Papers in international journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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nanomaterials-09-00567.pdf | 4,61 MB | Adobe PDF | Ver/Abrir |
Este trabalho está licenciado sob uma Licença Creative Commons