Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/60119
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Campo DC | Valor | Idioma |
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dc.contributor.author | Bacelo, Elisa | por |
dc.contributor.author | Alves da Silva, Marta | por |
dc.contributor.author | Cunha, Cristina | por |
dc.contributor.author | Faria, Susana | por |
dc.contributor.author | Carvalho, Agostinho | por |
dc.contributor.author | Reis, R. L. | por |
dc.contributor.author | Martins, Albino | por |
dc.contributor.author | Neves, Nuno M. | por |
dc.date.accessioned | 2019-04-17T11:19:50Z | - |
dc.date.available | 2019-04-17T11:19:50Z | - |
dc.date.issued | 2019-04-08 | - |
dc.identifier.issn | 2079-4991 | por |
dc.identifier.uri | https://hdl.handle.net/1822/60119 | - |
dc.description.abstract | Rheumatoid arthritis (RA) is an autoimmune disease that affects the synovial cavity of joints, and its pathogenesis is associated with an increased expression of pro-inflammatory cytokines, namely tumour necrosis factor-alpha (TNF-α). It has been clinically shown to have an adequate response to systemic administration of TNF-α inhibitors, although with many shortcomings. To overcome such limitations, the immobilization of a TNF-α antibody on a nanofibrous substrate to promote a localized action is herein proposed. By using this approach, the antibody has its maximum therapeutic efficacy and a prolonged therapeutic benefit, avoiding the systemic side-effects associated with conventional biological agents’ therapies. To technically achieve such a purpose, the surface of electrospun nanofibers is initially activated and functionalized, allowing TNF-α antibody immobilization at a maximum concentration of 6 µg/mL. Experimental results evidence that the biofunctionalized nanofibrous substrate is effective in achieving a sustained capture of soluble TNF-α over time. Moreover, cell biology assays demonstrate that this system has no deleterious effect over human articular chondrocytes metabolism and activity. Therefore, the developed TNF-capturing system may represent a potential therapeutic approach for the local management of severely affected joints. | por |
dc.description.sponsorship | This research was funded by the Portuguese Foundation for Science and Technology (FCT), namely the Post-doc fellowships of Marta Alves da Silva and Cristina Cunha (SFRH/BPD/73322/2010 and SFRH/BPD/96176/2013, respectively) and the Starting Investigator Grant of Agostinho Carvalho and Albino Martins (IF/00735/2014 and IF/00376/2014, respectively). It was also acknowledged the funded projects SPARTAN (PTDC/CTM-BIO/4388/2014) and FROnTHERA (NORTE-01-0145-FEDER-000023). | por |
dc.language.iso | eng | por |
dc.publisher | Multidisciplinary Digital Publishing Institute | por |
dc.relation | info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F73322%2F2010/PT | por |
dc.relation | info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F96176%2F2013/PT | por |
dc.relation | PTDC/CTM-BIO/4388/2014 | por |
dc.rights | openAccess | por |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | por |
dc.subject | antibody immobilization | por |
dc.subject | electrospun nanofibers | por |
dc.subject | TNF- capture | por |
dc.subject | human articular chondrocytes | por |
dc.subject | rheumatoid arthritis | por |
dc.subject | TNF-α capture | por |
dc.title | Biofunctional nanofibrous substrate for local TNF-capturing as a strategy to control inflammation in arthritic joints | por |
dc.type | article | por |
dc.peerreviewed | yes | por |
oaire.citationIssue | 4 | por |
oaire.citationVolume | 9 | por |
dc.date.updated | 2019-04-11T11:09:22Z | - |
dc.identifier.doi | 10.3390/nano9040567 | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Nanomaterials | por |
Aparece nas coleções: | ICVS - Artigos em revistas internacionais / Papers in international journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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nanomaterials-09-00567.pdf | 4,61 MB | Adobe PDF | Ver/Abrir |
Este trabalho está licenciado sob uma Licença Creative Commons