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|Title:||New perspectives of Juglans regia L. phytochemicals against Candida species|
Carvalho, Ana Maria
Silva, Sónia Carina
Ferreira, Isabel C. F. R.
Juglans regia L.
|Publisher:||European Society of Clinical Microbiology and Infectious Diseases (ESCMID)|
|Citation:||Martins, Natália; Barros, Lillian; Carvalho, Ana Maria; Silva, Sónia Carina; Henriques, Mariana; Ferreira, Isabel C. F. R., New perspectives of Juglans regia L. phytochemicals against Candida species. TEMPH2014 - Trends in Environmental Microbiology for Public Health. Lisboa, Portugal, Sep. 18-21, European Society of Clinical Microbiology and Infectious Diseases - ESCMID, 2014.|
|Abstract(s):||Opportunistic fungal infections have deserved special relevance in the last decades, presenting itself, a serious problem in terms of public health. Despite Candida albicans was considered the main agent responsible for those infections, other non-albicans Candida species have also been described in the last years [1-3]. Most of the species are susceptible to antimicrobial drugs, but recently it has been observed a growing number of microorganisms with drug resistance. Therefore, the discover/use of alternative therapies is crucial . Juglans regia L. (walnut) leaves are commonly used in traditional medicine as antiseptic, antimicrobial and anti-inflammatory ; those benefits could be related with its richness in phenolic compounds . In the present work, the antifungal potential of the hydroalcoholic extract prepared from walnut leaves was evaluated against a total of nineteen Candida strains (from the species: C.albicans, C.glabrata, C.parapsilosis and C.tropicalis), using the disc diffusion halo assay. All the tested strains were sensible to the plant extract. The obtained values of the inhibitory zones ranged between 0.9-1.4 cm, being the halo maintained after 48h. The observed antifungal activity is certainly related to the phenolic compounds previously determined in the extract : five phenolic acid derivatives- caffeoylquinic and p-coumaroylquinic acid derivatives, two dimers and one trimer of procyanidins, twelve flavonols- quercetin, myricetin and kaempferol derivatives, and five taxifolin O-pentoside isomers; 3-O-caffeoylquinic acids and quercetin O-pentoside were the main phenolic compounds. Further studies are necessaries in order to elucidate the most active compounds and the specific role of each one. REFERENCES  Kim J, Sudbery P. (2011) J Microbiol. 49, 171-177.  Tsai P-W, Chen Y-T, Hsu P-C, Lan C-Y. (2013) BioMedicine. 3, 51–64.  Vázquez-González D, Perusquía-Ortiz AM, Hundeiker M, Bonifaz A. (2013) J Ger Soc Dermatology. 11, 381–94.  Kanafani ZA, Perfect JR. (2008) Clin Infect Dis - Antimicrob Resist. 46, 120–128.  Murray MT. (2004) 2nd ed. New York, NY. Random House.  Santos A, Barros L, Calhelha RC, Dueñas M, Carvalho AM, Santos-Buelga C, Ferreira ICFR. (2013) Ind Crops Prod. 51, 430-436.|
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