Pulsed electromagnetic field modulates tendon cells response in il‐1β‐conditioned environment

Abstract

Strategies aiming at controlling and modulating inflammatory cues may offer therapeutic solutions for improving tendon regeneration. This study aims to investigate the modulatory effect of pulsed electromagnetic field (PEMF) on the inflammatory profile of human tendonâ derived cells (hTDCs) after supplementation with interleukinâ 1β (ILâ 1β). ILâ 1β was used to artificially induce in-flammatory cues associated with injured tendon environments. The PEMF effect was investigated varying the frequency (5 or 17 Hz), intensity (1.5, 4, or 5 mT), and dutyâ cycle (10% or 50%) parameters to which ILâ 1βâ treated hTDCs were exposed to. A PEMF actuation with 4 mT, 5 Hz and a 50% duty cycle decreased the production of ILâ 6 and tumor necrosis factorâ α (TNFâ α), as well as the expression of TNFα, ILâ 6, ILâ 8, COXâ 2, MMPâ 1, MMPâ 2, and MMPâ 3, while ILâ 4, ILâ 10, and TIMPâ 1 expression increased. These results suggest that PEMF stimulation can modulate hTDCs response in an inflammatory environment holding therapeutic potential for tendon regenerative strategies.