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TitlePartitioning of glycomacropeptide in aqueous two-phase systems
Author(s)Silva, C. A. S.
Coimbra, Jane Sélia dos Reis
Rojas, Edwin Elard Garcia
Teixeira, J. A.
KeywordsAqueous two-phase systems
PEG molar mass
NaCl addition
Tie line length
Issue dateNov-2009
PublisherElsevier Ltd.
JournalProcess Biochemistry
CitationSILVA, C. A. S. [et al.] - Partitioning of glycomacropeptide in aqueous two-phase systems. “Process Biochemistry” [Em linha]. 44:11 (Nov. 2009) 1213-1216. [Consult. 22 Set. 2009]. Disponível em WWW:<URL: ob=ArticleURL&_udi=B6THB-4WN2XW2-2&_ user=2459786&_rdoc=1&_fmt=&_orig=search&_ sort=d&_docanchor=&view=c&_searchStrId= 1020143195&_rerunOrigin=google&_acct= C000057396&_version=1&_urlVersion= 0&_userid=2459786&md5=d0a9d0a04be8a a0613e49a663eaeac7a>. ISSN 1359-5113.
Abstract(s)The partition behavior of glycomacropeptide (GMP) was determined in polyethylene glycol (PEG) and sodium citrate aqueous two-phase systems (ATPS). It was found that the partitioning of GMP depends on PEG molar mass, tie line length, pH, NaCl concentration and temperature. The obtained data indicates that GMP is preferentially partitioned into the PEG phase without addition of NaCl at pH 8.0. Larger tie line lengths and higher temperatures favor GMP partition to the PEG phase. Furthermore, it was verified that PEG molar mass and concentration have a slight effect on GMP partition. The increase in the molar mass of PEG induces a reduction of the protein solubility in the top PEG rich phase, being shown that the use of PEG1500 is beneficial for the extraction of GMP. A protein recovery higher than 85% was obtained in the top phase of these systems, clearly demonstrating its suitability as a starting point for the separation of GMP.
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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