Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/9612

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dc.contributor.authorSilva, C. A. S.-
dc.contributor.authorCoimbra, Jane Sélia dos Reis-
dc.contributor.authorRojas, Edwin Elard Garcia-
dc.contributor.authorTeixeira, J. A.-
dc.date.accessioned2009-09-22T15:55:51Z-
dc.date.available2009-09-22T15:55:51Z-
dc.date.issued2009-11-
dc.identifier.citationSILVA, C. A. S. [et al.] - Partitioning of glycomacropeptide in aqueous two-phase systems. “Process Biochemistry” [Em linha]. 44:11 (Nov. 2009) 1213-1216. [Consult. 22 Set. 2009]. Disponível em WWW:<URL:http://www.sciencedirect.com/science?_ ob=ArticleURL&_udi=B6THB-4WN2XW2-2&_ user=2459786&_rdoc=1&_fmt=&_orig=search&_ sort=d&_docanchor=&view=c&_searchStrId= 1020143195&_rerunOrigin=google&_acct= C000057396&_version=1&_urlVersion= 0&_userid=2459786&md5=d0a9d0a04be8a a0613e49a663eaeac7a>. ISSN 1359-5113.por
dc.identifier.issn1359-5113por
dc.identifier.urihttp://hdl.handle.net/1822/9612-
dc.description.abstractThe partition behavior of glycomacropeptide (GMP) was determined in polyethylene glycol (PEG) and sodium citrate aqueous two-phase systems (ATPS). It was found that the partitioning of GMP depends on PEG molar mass, tie line length, pH, NaCl concentration and temperature. The obtained data indicates that GMP is preferentially partitioned into the PEG phase without addition of NaCl at pH 8.0. Larger tie line lengths and higher temperatures favor GMP partition to the PEG phase. Furthermore, it was verified that PEG molar mass and concentration have a slight effect on GMP partition. The increase in the molar mass of PEG induces a reduction of the protein solubility in the top PEG rich phase, being shown that the use of PEG1500 is beneficial for the extraction of GMP. A protein recovery higher than 85% was obtained in the top phase of these systems, clearly demonstrating its suitability as a starting point for the separation of GMP.por
dc.description.sponsorshipResearch Support Foundation of Minas Gerais (FAPEMIG)por
dc.description.sponsorshipNational Council of Technological and Scientific Development (CNPq)por
dc.language.isoengpor
dc.publisherElsevier Ltd.por
dc.rightsopenAccesspor
dc.subjectAqueous two-phase systemspor
dc.subjectPEG molar masspor
dc.subjectNaCl additionpor
dc.subjectTie line lengthpor
dc.subjectpHpor
dc.titlePartitioning of glycomacropeptide in aqueous two-phase systemspor
dc.typearticlepor
dc.peerreviewedyespor
sdum.number11por
sdum.pagination1213–1216por
sdum.publicationstatuspublishedpor
sdum.volume44por
oaire.citationStartPage1213por
oaire.citationEndPage1216por
oaire.citationIssue11por
oaire.citationVolume44por
dc.identifier.doi10.1016/j.procbio.2009.06.016por
dc.subject.wosScience & Technologypor
sdum.journalProcess Biochemistrypor
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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