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https://hdl.handle.net/1822/85285
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Campo DC | Valor | Idioma |
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dc.contributor.author | Magalhães, Andreia Patrícia Alves | por |
dc.contributor.author | Graínha, Tânia Raquel Rodrigues | por |
dc.contributor.author | Sousa, Ana Margarida | por |
dc.contributor.author | França, Ângela Maria Oliveira Sousa | por |
dc.contributor.author | Cerca, Nuno | por |
dc.contributor.author | Pereira, Maria Olívia | por |
dc.date.accessioned | 2023-06-30T17:13:22Z | - |
dc.date.available | 2023-07-01T06:00:32Z | - |
dc.date.issued | 2019-09-05 | - |
dc.identifier.citation | Magalhães, Andreia P.; Grainha, Tânia; Sousa, Ana Margarida; França, Angela; Cerca, Nuno; Pereira, Maria Olívia, Viable but non-culturable state: a strategy for Staphylococcus aureus survivable in dual-species biofilms with Pseudomonas aeruginosa. EUROBIOFILMS 2019 - 6th European Congress on Biofilms. No. 164, Glasgow, Scotland, Sep 03-06, 67, 2019. | por |
dc.identifier.uri | https://hdl.handle.net/1822/85285 | - |
dc.description.abstract | While considerable research has focused on studying individual-species, we now face the challenge of determining how interspecies interactions alter bacterial behaviours and pathogenesis. Of particular interest is the ecological behaviour between P. aeruginosa-S. aureus, since microbial communities containing both pathogens can display enhanced virulence. Curiously, their interaction is often reported as competitive under laboratory conditions. Here we investigate the interaction between P. aeruginosa-S. aureus in a biofilm co-culture model. The growth of 24- and 48h-old dual-species biofilms was monitored by CFU and flow-cytometry counting, and the gene expression profile inspected by qPCR. Plating experiments showed that S. aureus growth significantly decreased from 24- up to 72-h of growth in dual-species biofilms with P. aeruginosa, suggesting a clear competitive advantage for P. aeruginosa after 24-h. The gene expression profile showed that P. aeruginosa PA14 significantly overexpressed several key virulence-related genes (pqsE, rhlR, pvdE and lasI) after 24-h of growth with S. aureus ATCC 25923, comparatively with singles-pecies biofilms, which could justify the rapid decline of S. aureus population after 24-h of co-culture with P. aeruginosa. Nevertheless, the isolated strain P. aeruginosa 362668 non-mucoid exhibited a reverse pattern by downregulated the expression of those genes during dual-species growth. As P. aeruginosa exoproducts in addition to cause S. aureus lysis or inhibition may also induce biofilm dispersion, the bulk-fluid of 48-h biofilms was analysed by culture counts and flow-cytometry. Interestingly, while no S. aureus cells were detected by CFU, flow-cytometry data revealed the presence of S. aureus in high numbers (~6 Log). The metabolic activity of S. aureus cells was confirmed by measure the expression levels of sodA gene. In conclusion, the presence of S. aureus in a viable but non-cultivable state within dual-species biofilms with P. aeruginosa underscores the impact of interspecies interactions on antibiotic efficacy in the context of polymicrobial infections. | por |
dc.description.sponsorship | Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2019 unit; European Regional Development Fund under the scope of Norte2020 - Programa Operacional Regional do Norte for the BioTecNorte operation (NORTE-01-0145-FEDER-000004); COMPETE2020 and FCT for the project POCI-01-0145-FEDER-029841. The authors also thank FCT for the PhD Grant of Andreia Patrícia Magalhães [grant number SFRH/BD/132165/2017] | por |
dc.language.iso | eng | por |
dc.relation | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FBIO%2F04469%2F2019/PT | - |
dc.rights | openAccess | por |
dc.title | Viable but non-culturable state: a strategy for Staphylococcus aureus survivable in dual-species biofilms with Pseudomonas aeruginosa | por |
dc.type | panelPresentation | por |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://www.eurobiofilms2019.com | por |
dc.comments | CEB52084 | por |
oaire.citationStartPage | 67 | por |
oaire.citationIssue | 164 | por |
oaire.citationConferencePlace | Glasgow, Scotland | por |
dc.date.updated | 2023-06-26T09:49:05Z | - |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | - |
sdum.conferencePublication | EUROBIOFILMS 2019 - 6th European Congress on Biofilms | por |
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Ficheiro | Descrição | Tamanho | Formato | |
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document_52084_1.pdf | Abstract | 45,34 kB | Adobe PDF | Ver/Abrir |
document_52084_2.pdf | Presentation | 68,99 MB | Adobe PDF | Ver/Abrir |