Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/75841
Título: | Unilateral intrastriatal 6-hydroxydopamine lesion in mice: a closer look into non-motor phenotype and glial response |
Autor(es): | Pinheiro, Bárbara Filipa Mendes Cunha, Carina Isabel Soares Marote, Ana Maria Franco Aveiro Loureiro-Campos, Eduardo Campos, Jonas Oliveira Barata-Antunes, Sandra Fernandes, Daniela Monteiro Santos, Diogo Jorge Ferreira Silva, Sara Carina Duarte Pinto, Luísa Salgado, A. J. |
Palavras-chave: | Parkinson’s disease 6-OHDA Unilateral Striatum Dopaminergic degeneration Motor behavior Non-motor behavior Glial response |
Data: | 26-Out-2021 |
Editora: | Multidisciplinary Digital Publishing Institute (MDPI) |
Revista: | International Journal of Molecular Sciences |
Citação: | Mendes-Pinheiro, B.; Soares-Cunha, C.; Marote, A.; Loureiro-Campos, E.; Campos, J.; Barata-Antunes, S.; Monteiro-Fernandes, D.; Santos, D.; Duarte-Silva, S.; Pinto, L.; José Salgado, A. Unilateral Intrastriatal 6-Hydroxydopamine Lesion in Mice: A Closer Look into Non-Motor Phenotype and Glial Response. Int. J. Mol. Sci. 2021, 22, 11530. https://doi.org/10.3390/ijms222111530 |
Resumo(s): | Parkinson’s disease (PD) is a prevalent movement disorder characterized by the progressive loss of dopaminergic neurons in substantia nigra pars compacta (SNpc). The 6-hydroxydopamine (6-OHDA) lesion is still one of the most widely used techniques for modeling Parkinson’s disease (PD) in rodents. Despite commonly used in rats, it can be challenging to reproduce a similar lesion in mice. Moreover, there is a lack of characterization of the extent of behavioral deficits and of the neuronal loss/neurotransmitter system in unilateral lesion mouse models. In this study, we present an extensive behavioral and histological characterization of a unilateral intrastriatal 6-OHDA mouse model. Our results indicate significant alterations in balance and fine motor coordination, voluntary locomotion, and in the asymmetry’s degree of forelimb use in 6-OHDA lesioned animals, accompanied by a decrease in self-care and motivational behavior, common features of depressive-like symptomatology. These results were accompanied by a decrease in tyrosine hydroxylase (TH)-labelling and dopamine levels within the nigrostriatal pathway. Additionally, we also identify a marked astrocytic reaction, as well as proliferative and reactive microglia in lesioned areas. These results confirm the use of unilateral intrastriatal 6-OHDA mice for the generation of a mild model of nigrostriatal degeneration and further evidences the recapitulation of key aspects of PD, thereby being suitable for future studies beholding new therapeutical interventions for this disease. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/75841 |
DOI: | 10.3390/ijms222111530 |
ISSN: | 1661-6596 |
e-ISSN: | 1422-0067 |
Versão da editora: | https://www.mdpi.com/1422-0067/22/21/11530 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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ijms-22-11530.pdf | 3,59 MB | Adobe PDF | Ver/Abrir |
Este trabalho está licenciado sob uma Licença Creative Commons