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https://hdl.handle.net/1822/75841
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Campo DC | Valor | Idioma |
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dc.contributor.author | Pinheiro, Bárbara Filipa Mendes | por |
dc.contributor.author | Cunha, Carina Isabel Soares | por |
dc.contributor.author | Marote, Ana Maria Franco Aveiro | por |
dc.contributor.author | Loureiro-Campos, Eduardo | por |
dc.contributor.author | Campos, Jonas Oliveira | por |
dc.contributor.author | Barata-Antunes, Sandra | por |
dc.contributor.author | Fernandes, Daniela Monteiro | por |
dc.contributor.author | Santos, Diogo Jorge Ferreira | por |
dc.contributor.author | Silva, Sara Carina Duarte | por |
dc.contributor.author | Pinto, Luísa | por |
dc.contributor.author | Salgado, A. J. | por |
dc.date.accessioned | 2022-02-09T15:34:17Z | - |
dc.date.available | 2022-02-09T15:34:17Z | - |
dc.date.issued | 2021-10-26 | - |
dc.identifier.citation | Mendes-Pinheiro, B.; Soares-Cunha, C.; Marote, A.; Loureiro-Campos, E.; Campos, J.; Barata-Antunes, S.; Monteiro-Fernandes, D.; Santos, D.; Duarte-Silva, S.; Pinto, L.; José Salgado, A. Unilateral Intrastriatal 6-Hydroxydopamine Lesion in Mice: A Closer Look into Non-Motor Phenotype and Glial Response. Int. J. Mol. Sci. 2021, 22, 11530. https://doi.org/10.3390/ijms222111530 | por |
dc.identifier.issn | 1661-6596 | - |
dc.identifier.uri | https://hdl.handle.net/1822/75841 | - |
dc.description.abstract | Parkinson’s disease (PD) is a prevalent movement disorder characterized by the progressive loss of dopaminergic neurons in substantia nigra pars compacta (SNpc). The 6-hydroxydopamine (6-OHDA) lesion is still one of the most widely used techniques for modeling Parkinson’s disease (PD) in rodents. Despite commonly used in rats, it can be challenging to reproduce a similar lesion in mice. Moreover, there is a lack of characterization of the extent of behavioral deficits and of the neuronal loss/neurotransmitter system in unilateral lesion mouse models. In this study, we present an extensive behavioral and histological characterization of a unilateral intrastriatal 6-OHDA mouse model. Our results indicate significant alterations in balance and fine motor coordination, voluntary locomotion, and in the asymmetry’s degree of forelimb use in 6-OHDA lesioned animals, accompanied by a decrease in self-care and motivational behavior, common features of depressive-like symptomatology. These results were accompanied by a decrease in tyrosine hydroxylase (TH)-labelling and dopamine levels within the nigrostriatal pathway. Additionally, we also identify a marked astrocytic reaction, as well as proliferative and reactive microglia in lesioned areas. These results confirm the use of unilateral intrastriatal 6-OHDA mice for the generation of a mild model of nigrostriatal degeneration and further evidences the recapitulation of key aspects of PD, thereby being suitable for future studies beholding new therapeutical interventions for this disease. | por |
dc.description.sponsorship | This work was supported by the Foundation for Science and Technology (FCT) through PhD Fellowships attributed to B.M.P. (SFRH/BD/120124/2016), E.L.C. (SFRH/BD/131278/2017), J.C. (SFRH/BD/5813/2020), S.B.A. (PD/BDE/135568/2018), D.M.F. (SFRH/BD/147947/2019), and CEEC attributed to C.S.C. (CEECIND/03887/2017), S.D.S. (CEECIND/00685/2020), and L.P. (CEECIND/ 02855/2020). ICVS Scientific Microscopy Platform, member of the national infrastructure PPBI— Portuguese Platform of Bioimaging PPBI-POCI-01-0145-FEDER-022122; National funds, through the FCT—project UIDB/50026/2020 and UIDP/50026/2020; the projects NORTE-01-0145-FEDER-000013 and NORTE-01-0145-FEDER-000023, supported by Norte Portugal Regional Operational Programme (NORTE 2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF); Competitiveness Internationalization Operational Programme (POCI), and by National funds, through the FCT, under the scope of the project POCI-01-0145-FEDER-032619. | por |
dc.language.iso | eng | por |
dc.publisher | Multidisciplinary Digital Publishing Institute (MDPI) | por |
dc.relation | SFRH/BD/120124/2016 | por |
dc.relation | SFRH/BD/131278/2017 | por |
dc.relation | SFRH/BD/5813/2020 | por |
dc.relation | PD/BDE/135568/2018 | por |
dc.relation | SFRH/BD/147947/2019 | por |
dc.relation | CEECIND/03887/2017 | por |
dc.relation | CEECIND/00685/2020 | por |
dc.relation | CEECIND/02855/2020 | por |
dc.relation | UIDB/50026/2020 | por |
dc.relation | UIDP/50026/2020 | por |
dc.rights | openAccess | por |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | por |
dc.subject | Parkinson’s disease | por |
dc.subject | 6-OHDA | por |
dc.subject | Unilateral | por |
dc.subject | Striatum | por |
dc.subject | Dopaminergic degeneration | por |
dc.subject | Motor behavior | por |
dc.subject | Non-motor behavior | por |
dc.subject | Glial response | por |
dc.title | Unilateral intrastriatal 6-hydroxydopamine lesion in mice: a closer look into non-motor phenotype and glial response | por |
dc.type | article | por |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://www.mdpi.com/1422-0067/22/21/11530 | por |
oaire.citationStartPage | 1 | por |
oaire.citationEndPage | 19 | por |
oaire.citationIssue | 21 | por |
oaire.citationVolume | 22 | por |
dc.date.updated | 2021-11-11T14:57:24Z | - |
dc.identifier.eissn | 1422-0067 | - |
dc.identifier.doi | 10.3390/ijms222111530 | por |
dc.identifier.pmid | 34768962 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | International Journal of Molecular Sciences | por |
oaire.version | VoR | por |
dc.identifier.articlenumber | 11530 | por |
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