Utilize este identificador para referenciar este registo: https://hdl.handle.net/1822/75153

TítuloBiocontrol of Pseudomonas syringae pv. actinidiae in kiwi plants using bacteriophages
Autor(es)Rego, Rute
Pires, Diana Priscila Penso
Monte, Rafaela
Azeredo, Joana
Santos, Conceição
Moura, Luísa
Data23-Nov-2021
CitaçãoRego, Rute; Pires, Diana P.; Monte, Rafaela; Azeredo, Joana; Santos, Conceição; Moura, Luísa, Biocontrol of Pseudomonas syringae pv. actinidiae in kiwi plants using bacteriophages. Microbiotec 21 - Congress of Microbiology and Biotechnology (Abstracts Book). No. 317, UNL Online, Nov 23-26, 700-701, 2021.
Resumo(s)Pseudomonas syringae pv. actinidiae (Psa) is the causal agent of bacterial canker in kiwifruit (Actinidia deliciosa), leading to severe symptoms in plants and consequent considerable production and financial losses. Current methods for controlling this disease rely on the use of copper-based products and, in countries outside the European Union, in the use of antibiotics. These products, besides being considered phytotoxic, can lead to the spread of resistance to both copper and antibiotics. With the increasing need to obtain safe and effective biocontrol strategies against this pathogen, this study focused on the isolation and characterization of (bacterio)phages for the control of Psa. A collection of Psa strains and phages were isolated from branches, buds, leaves, petals, sepals, and stamens from kiwifruit plants in orchards in the North of Portugal. Phages were isolated using the enrichment procedure with Psa strains CFBP 7286 and P84 as possible hosts, and the lytic spectra of 6 selected phages were tested against the Psa collection. Three phages displaying broader host ranges (between 90 to 97% of efficacy among Psa strains) were selected for further analysis. Phage stability was studied at different temperatures (-20º to 60ºC), pH (1-13) and UV light, and it was observed that the three phages were stable between -20ºC and 50ºC, pH range of 3 to 11 and UV light at 366 nm up to 180 min. The morphological characterization of phages was done by Transmission Electron Microscopy and the genome sequencing of these phages is ongoing. In vitro efficacy assays showed that phage 177T was able to reduce the number of CFUs after 4h of inoculation and keeping the bacterial load at low levels for up to 24h post-infection with MOI=1. In vitro efficacy tests were also carried out on kiwi leaf discs, and it was possible to detect a reduction in the bacterial load of Psa. Altogether, our results clearly highlight the potential of phages as promising biocontrol agents against Psa, that could be used in the future to replace copper compounds and antibiotics.
TipoResumo em ata de conferência
URIhttps://hdl.handle.net/1822/75153
Versão da editorahttps://microbiotec21.organideia.pt/
Arbitragem científicayes
AcessoAcesso aberto
Aparece nas coleções:CEB - Resumos em Livros de Atas / Abstracts in Proceedings

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