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TitleApplication of nucleic acid mimics in fluorescence in situ hybridization
Author(s)Oliveira, Ricardo
Azevedo, Andreia S.
Mendes, Luzia
Nucleic acid mimics
Issue date13-Feb-2021
JournalMethods in Molecular Biology
CitationOliveira, Ricardo; Azevedo, Andreia S.; Mendes, Luzia, Application of Nucleic Acid Mimics in Fluorescence In Situ Hybridization. In Nuno F. Azevedo, Carina Almeida, Fluorescence In-Situ Hybridization (FISH) for Microbial Cells: Methods and Concepts, Vol. Methods in Molecular Biology 2246, Springer US, 2021. ISBN: 978-1-0716-1115-9, 69-86. DOI: 10.1007/978-1-0716-1115-9_5
Abstract(s)Traditionally, RNA and DNA probes are used in fluorescence in situ hybridization (FISH) methods for microbial detection and characterization of communities structure and diversity. However, the recent introduction of nucleic acid mimics (NAMs) has improved the robustness of the FISH methods in terms of sensitivity and specificity. Several NAMs have been used, of which the most relevant are peptide nucleic acid (PNA), locked nucleic acids (LNA), 2-O-methyl RNA (2OMe), and phosphorothioates (PS). In this chapter, we describe a protocol using PNA and LNA/2OMe probes for microbial detection by FISH, pointing out the differences between them. These protocols are easily adapted to different microorganisms and different probe sequences.
TypeBook part
Publisher version
AccessRestricted access (UMinho)
Appears in Collections:CEB - Livros e Capítulos de Livros / Books and Book Chapters

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