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https://hdl.handle.net/1822/74497| Título: | Characterization of social interactions and spatial arrangement of individual bacteria in multistrain or multispecies biofilm systems using nucleic acid mimics-fluorescence in situ hybridization |
| Autor(es): | Allkja, Jontana Azevedo, Andreia S. |
| Palavras-chave: | FISH Nucleic acid mimics PNA LNA 2OMe Bacteria Biofilm Multispecies Multistrain Microscopy CLSM |
| Data: | 13-Fev-2021 |
| Editora: | Springer |
| Revista: | Methods in Molecular Biology |
| Citação: | Allkja, Jontana; Azevedo, Andreia S., Characterization of social interactions and spatial arrangement of individual bacteria in multistrain or multispecies biofilm systems using nucleic acid mimics-fluorescence in situ hybridization. In Nuno F. Azevedo, Carina Almeida, Fluorescence In-Situ Hybridization (FISH) for Microbial Cells: Methods and Concepts, Vol. Methods in Molecular Biology 2246, Springer US, 2021. ISBN: 978-1-0716-1115-9, 97-109 |
| Resumo(s): | Biofilms are often composed of different bacterial and fungal species/strains, which form complex structures based on social interactions with each other. Fluorescence in situ hybridization (FISH) can help us identify the different species/strains present within a biofilm, and when coupled with confocal scanning laser microscopy (CSLM), it enables the visualization of the three-dimensional (3D) structure of the biofilm and the spatial arrangement of each individual species/strain within it. In this chapter, we describe the protocol for characterizing multistrain or multispecies biofilm formation using NAM-FISH and CSLM. |
| Tipo: | Capítulo de livro |
| URI: | https://hdl.handle.net/1822/74497 |
| ISBN: | 978-1-0716-1115-9 |
| DOI: | 10.1007/978-1-0716-1115-9_7 |
| ISSN: | 1064-3745 |
| Versão da editora: | https://link.springer.com/book/10.1007/978-1-0716-1115-9 |
| Arbitragem científica: | yes |
| Acesso: | Acesso restrito UMinho |
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| document_54198_1.pdf Acesso restrito! | 376,05 kB | Adobe PDF | Ver/Abrir |
