Please use this identifier to cite or link to this item: https://hdl.handle.net/1822/74497

TitleCharacterization of social interactions and spatial arrangement of individual bacteria in multistrain or multispecies biofilm systems using nucleic acid mimics-fluorescence in situ hybridization
Author(s)Allkja, Jontana
Azevedo, Andreia S.
KeywordsFISH
Nucleic acid mimics
PNA
LNA
2OMe
Bacteria
Biofilm
Multispecies
Multistrain
Microscopy
CLSM
Issue date13-Feb-2021
PublisherSpringer
JournalMethods in Molecular Biology
CitationAllkja, Jontana; Azevedo, Andreia S., Characterization of social interactions and spatial arrangement of individual bacteria in multistrain or multispecies biofilm systems using nucleic acid mimics-fluorescence in situ hybridization. In Nuno F. Azevedo, Carina Almeida, Fluorescence In-Situ Hybridization (FISH) for Microbial Cells: Methods and Concepts, Vol. Methods in Molecular Biology 2246, Springer US, 2021. ISBN: 978-1-0716-1115-9, 97-109
Abstract(s)Biofilms are often composed of different bacterial and fungal species/strains, which form complex structures based on social interactions with each other. Fluorescence in situ hybridization (FISH) can help us identify the different species/strains present within a biofilm, and when coupled with confocal scanning laser microscopy (CSLM), it enables the visualization of the three-dimensional (3D) structure of the biofilm and the spatial arrangement of each individual species/strain within it. In this chapter, we describe the protocol for characterizing multistrain or multispecies biofilm formation using NAM-FISH and CSLM.
TypeBook part
URIhttps://hdl.handle.net/1822/74497
ISBN978-1-0716-1115-9
DOI10.1007/978-1-0716-1115-9_7
ISSN1064-3745
Publisher versionhttps://link.springer.com/book/10.1007/978-1-0716-1115-9
Peer-Reviewedyes
AccessRestricted access (UMinho)
Appears in Collections:CEB - Livros e Capítulos de Livros / Books and Book Chapters

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