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TitleFlow-FISH using nucleic acid mimic probes for the detection of bacteria
Author(s)Azevedo, Andreia S.
Rocha, Rui
Dias, Nicolina Marques
KeywordsPeptide nucleic acid
Locked nucleic acid
2'O-methyl RNA
Nucleic acid mimics
Flow cytometry
Issue date13-Feb-2021
JournalMethods in Molecular Biology
CitationAzevedo, Andreia S.; Rocha, Rui; Dias, Nicolina M., Flow-FISH using nucleic acid mimic probes for the detection of bacteria. In Nuno F. Azevedo, Carina Almeida, Fluorescence In-Situ Hybridization (FISH) for Microbial Cells: Methods and Concepts, Vol. Methods in Molecular Biology 2246, Springer US, 2021. ISBN: 978-1-0716-1115-9, 263-277
Abstract(s)Flow-Fluorescence in situ hybridization (Flow-FISH) enables multiparametric high-throughput detection of target nucleic acid sequences at the single cell-level, allowing an accurate quantification of different cell populations by using a combination of flow cytometry and fluorescent in situ hybridization (FISH). In this chapter, a flow-FISH protocol is described with labeled nucleic acid mimics (NAMs) (e.g. LNA/2OMe and PNA) acting as the reporter molecules. This protocol allows for the specific detection of bacterial cells. Hence, this protocol can be carried out with minor adjustments, in order to simultaneously detect different species of bacteria in different types of clinical, food, or environmental samples.
TypeBook part
Publisher version
AccessRestricted access (UMinho)
Appears in Collections:CEB - Livros e Capítulos de Livros / Books and Book Chapters

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