Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/74354
Título: | 3D-printed gelatin methacrylate scaffolds with controlled architecture and stiffness modulate the fibroblast phenotype towards dermal regeneration |
Autor(es): | R. Ibañez, Rita I. do Amaral, Ronaldo J. F. C. Reis, R. L. Marques, A. P. Murphy, Ciara M. O’Brien, Fergal J. |
Palavras-chave: | Biomaterial stiffness Porosity Wound healing GelMA 3D printing Fibroblast Fibrosis inhibition |
Data: | 30-Jul-2021 |
Editora: | Multidisciplinary Digital Publishing Institute (MDPI) |
Revista: | Polymers |
Citação: | R. Ibañez, R.I.; do Amaral, R.J.F.C.; Reis, R.L.; Marques, A.P.; Murphy, C.M.; O’Brien, F.J. 3D-Printed Gelatin Methacrylate Scaffolds with Controlled Architecture and Stiffness Modulate the Fibroblast Phenotype towards Dermal Regeneration. Polymers 2021, 13, 2510. https://doi.org/10.3390/polym13152510 |
Resumo(s): | Impaired skin wound healing due to severe injury often leads to dysfunctional scar tissue formation as a result of excessive and persistent myofibroblast activation, characterised by the increased expression of α-smooth muscle actin (αSMA) and extracellular matrix (ECM) proteins. Yet, despite extensive research on impaired wound healing and the advancement in tissue-engineered skin substitutes, scar formation remains a significant clinical challenge. This study aimed to first investigate the effect of methacrylate gelatin (GelMA) biomaterial stiffness on human dermal fibroblast behaviour in order to then design a range of 3D-printed GelMA scaffolds with tuneable structural and mechanical properties and understand whether the introduction of pores and porosity would support fibroblast activity, while inhibiting myofibroblast-related gene and protein expression. Results demonstrated that increasing GelMA stiffness promotes myofibroblast activation through increased fibrosis-related gene and protein expression. However, the introduction of a porous architecture by 3D printing facilitated healthy fibroblast activity, while inhibiting myofibroblast activation. A significant reduction was observed in the gene and protein production of αSMA and the expression of ECM-related proteins, including fibronectin I and collagen III, across the range of porous 3D-printed GelMA scaffolds. These results show that the 3D-printed GelMA scaffolds have the potential to improve dermal skin healing, whilst inhibiting fibrosis and scar formation, therefore potentially offering a new treatment for skin repair. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/74354 |
DOI: | 10.3390/polym13152510 |
e-ISSN: | 2073-4360 |
Versão da editora: | https://www.mdpi.com/2073-4360/13/15/2510 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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polymers-13-02510-v2.pdf | 4,56 MB | Adobe PDF | Ver/Abrir |
Este trabalho está licenciado sob uma Licença Creative Commons