Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/74354
Registo completo
Campo DC | Valor | Idioma |
---|---|---|
dc.contributor.author | R. Ibañez, Rita I. | por |
dc.contributor.author | do Amaral, Ronaldo J. F. C. | por |
dc.contributor.author | Reis, R. L. | por |
dc.contributor.author | Marques, A. P. | por |
dc.contributor.author | Murphy, Ciara M. | por |
dc.contributor.author | O’Brien, Fergal J. | por |
dc.date.accessioned | 2021-10-14T10:17:55Z | - |
dc.date.available | 2021-10-14T10:17:55Z | - |
dc.date.issued | 2021-07-30 | - |
dc.identifier.citation | R. Ibañez, R.I.; do Amaral, R.J.F.C.; Reis, R.L.; Marques, A.P.; Murphy, C.M.; O’Brien, F.J. 3D-Printed Gelatin Methacrylate Scaffolds with Controlled Architecture and Stiffness Modulate the Fibroblast Phenotype towards Dermal Regeneration. Polymers 2021, 13, 2510. https://doi.org/10.3390/polym13152510 | por |
dc.identifier.uri | https://hdl.handle.net/1822/74354 | - |
dc.description.abstract | Impaired skin wound healing due to severe injury often leads to dysfunctional scar tissue formation as a result of excessive and persistent myofibroblast activation, characterised by the increased expression of α-smooth muscle actin (αSMA) and extracellular matrix (ECM) proteins. Yet, despite extensive research on impaired wound healing and the advancement in tissue-engineered skin substitutes, scar formation remains a significant clinical challenge. This study aimed to first investigate the effect of methacrylate gelatin (GelMA) biomaterial stiffness on human dermal fibroblast behaviour in order to then design a range of 3D-printed GelMA scaffolds with tuneable structural and mechanical properties and understand whether the introduction of pores and porosity would support fibroblast activity, while inhibiting myofibroblast-related gene and protein expression. Results demonstrated that increasing GelMA stiffness promotes myofibroblast activation through increased fibrosis-related gene and protein expression. However, the introduction of a porous architecture by 3D printing facilitated healthy fibroblast activity, while inhibiting myofibroblast activation. A significant reduction was observed in the gene and protein production of αSMA and the expression of ECM-related proteins, including fibronectin I and collagen III, across the range of porous 3D-printed GelMA scaffolds. These results show that the 3D-printed GelMA scaffolds have the potential to improve dermal skin healing, whilst inhibiting fibrosis and scar formation, therefore potentially offering a new treatment for skin repair. | por |
dc.description.sponsorship | The authors acknowledge funding from Science Foundation Ireland under the M-ERA.NET program, Transnational Call 2016 (17/US/3437; Ireland), EU BlueHuman Interreg Atlantic Area Project (grant EAPA_151/2016) and Science Foundation Ireland, through the Advanced Materials and BioEngineering Research Centre (AMBER; grants 12/RC/2278 and 12/RC/2278_P2). | por |
dc.language.iso | eng | por |
dc.publisher | Multidisciplinary Digital Publishing Institute (MDPI) | por |
dc.rights | openAccess | por |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | por |
dc.subject | Biomaterial stiffness | por |
dc.subject | Porosity | por |
dc.subject | Wound healing | por |
dc.subject | GelMA | por |
dc.subject | 3D printing | por |
dc.subject | Fibroblast | por |
dc.subject | Fibrosis inhibition | por |
dc.title | 3D-printed gelatin methacrylate scaffolds with controlled architecture and stiffness modulate the fibroblast phenotype towards dermal regeneration | por |
dc.type | article | por |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://www.mdpi.com/2073-4360/13/15/2510 | por |
oaire.citationStartPage | 1 | por |
oaire.citationEndPage | 21 | por |
oaire.citationIssue | 15 | por |
oaire.citationVolume | 13 | por |
dc.date.updated | 2021-08-06T15:19:11Z | - |
dc.identifier.eissn | 2073-4360 | - |
dc.identifier.doi | 10.3390/polym13152510 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Polymers | por |
oaire.version | VoR | por |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
---|---|---|---|---|
polymers-13-02510-v2.pdf | 4,56 MB | Adobe PDF | Ver/Abrir |
Este trabalho está licenciado sob uma Licença Creative Commons