Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/67703
Título: | NEDD8: a new ataxin-3 interactor |
Autor(es): | Ferro, Anabela Carvalho, Ana Luísa Castro, Andreia Cristiana Teixeira Almeida, Carla Tomé, Ricardo J. Cortes, Luísa Rodrigues, Ana João Santos, Elsa Clara Carvalho Logarinho Sequeiros, Jorge Maciel, P. |
Palavras-chave: | Animals Ataxin-3 Binding sites HeLa cells Humans Hydrolysis Mammals Models, Molecular NEDD8 protein Nerve tissue proteins Nuclear proteins Protein binding Protein transport Repressor proteins Substrate specificity Two-Hybrid system techniques Ubiquitin Polyglutamine UBL E3 ligase Neurodegeneration MJD/SCA3 |
Data: | Nov-2007 |
Editora: | Elsevier 1 |
Revista: | Biochimica et Biophysica Acta (BBA). Molecular Cell Research |
Resumo(s): | Machado-Joseph disease (MJD/SCA3) is an autosomal dominant neurodegenerative disease caused by the expansion of a CAG tract in the coding portion of the ATXN3 gene. The presence of ubiquitin-positive aggregates of the defective protein in affected neurons is characteristic of this and most of the polyglutamine disorders. Recently, the accumulation of the neural precursor cell expressed developmentally downregulated 8 (NEDD8), a ubiquitin-like protein, in the inclusions of MJD brains was reported. Here, we report a new molecular interaction between wild-type ataxin-3 and NEDD8, using in vitro and in situ approaches. Furthermore, we show that this interaction is not dependent on the ubiquitin-interacting motifs in ataxin-3, since the presence of the Josephin domain is sufficient for the interaction to occur. The conservation of the interaction between the Caenorhabditis elegans ataxin-3 homologue (atx-3) and NEDD8 suggests its biological and functional relevance. Molecular docking studies of the NEDD8 molecule to the Josephin domain of ataxin-3 suggest that NEDD8 interacts with ataxin-3 in a substrate-like mode. In agreement, ataxin-3 displays deneddylase activity against a fluorogenic NEDD8 substrate. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/67703 |
DOI: | 10.1016/j.bbamcr.2007.07.012 |
ISSN: | 0167-4889 |
e-ISSN: | 1879-2596 |
Versão da editora: | https://www.sciencedirect.com/science/article/pii/S0167488907001917 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | ICVS - Artigos em revistas internacionais / Papers in international journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Ferro-2007-Nedd-a-new-ataxin--interactor.pdf | 909,23 kB | Adobe PDF | Ver/Abrir |