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https://hdl.handle.net/1822/67248
Título: | Overexpression of circulating MiR-30b-5p identifies advanced breast cancer |
Autor(es): | Estevão-Pereira, Helena Lobo, João Salta, Sofia Amorim, Maria Lopes, Paula Cantante, Mariana Reis, Berta Antunes, Luís Castro, Fernando Palma de Sousa, Susana Gonçalves, Céline S. Costa, Bruno Marques Henrique, Rui Jerónimo, Carmen |
Palavras-chave: | Adult Aged Biomarkers, Tumor Breast Neoplasms Circulating MicroRNA Cohort Studies Female Humans Liquid Biopsy MicroRNAs Middle Aged Neoplasm Staging Prognosis ROC Curve Gene Expression Regulation, Neoplastic Breast cancer Metastasis Biomarkers |
Data: | 2019 |
Editora: | BioMed Central (BMC) |
Revista: | Journal of Translational Medicine |
Citação: | Estevão-Pereira, H., Lobo, J., Salta, S., Amorim, M., et. al. (2019). Overexpression of circulating MiR-30b-5p identifies advanced breast cancer. Journal of translational medicine, 17(1), 435 |
Resumo(s): | Breast cancer (BrC) remains the leading cause of cancer-related death in women, mainly due to recurrent and/or metastatic events, entailing the need for biomarkers predictive of progression to advanced disease. MicroRNAs hold promise as noninvasive cancer biomarkers due to their inherent stability and resilience in tissues and bodily fluids. There is increasing evidence that specific microRNAs play a functional role at different steps of the metastatic cascade, behaving as signaling mediators to enable the colonization of a specific organ. Herein, we aimed to evaluate the biomarker performance of microRNAs previously reported as associated with prognosis for predicting BrC progression in liquid biopsies. Background Breast cancer (BrC) remains the leading cause of cancer-related death in women, mainly due to recurrent and/or metastatic events, entailing the need for biomarkers predictive of progression to advanced disease. MicroRNAs hold promise as noninvasive cancer biomarkers due to their inherent stability and resilience in tissues and bodily fluids. There is increasing evidence that specific microRNAs play a functional role at different steps of the metastatic cascade, behaving as signaling mediators to enable the colonization of a specific organ. Herein, we aimed to evaluate the biomarker performance of microRNAs previously reported as associated with prognosis for predicting BrC progression in liquid biopsies. Methods Selected microRNAs were assessed using a quantitative reverse transcription-polymerase chain reaction in a testing cohort of formalin-fixed paraffin-embedded primary (n = 16) and metastatic BrC tissues (n = 22). Then, miR-30b-5p and miR-200b-3p were assessed in a validation cohort #1 of formalin-fixed paraffin-embedded primary (n = 82) and metastatic BrC tissues (n = 93), whereas only miR-30b-5p was validated on a validation cohort #2 of liquid biopsies from BrC patients with localized (n = 20) and advanced (n = 25) disease. ROC curve was constructed to evaluate prognostic performance. Results MiR-30b-5p was differentially expressed in primary tumors and paired metastatic lesions, with bone metastases displaying significantly higher miR-30b-5p expression levels, paralleling the corresponding primary tumors. Interestingly, patients with advanced disease disclosed increased circulating miR-30b-5p expression compared to patients with localized BrC. Conclusions MiR-30b-5p might identify BrC patients at higher risk of disease progression, thus, providing a useful clinical tool for patients’ monitoring, entailing earlier and more effective treatment. Nonetheless, validation in larger multicentric cohorts is mandatory to confirm these findings. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/67248 |
DOI: | 10.1186/s12967-019-02193-y |
e-ISSN: | 1479-5876 |
Versão da editora: | https://link.springer.com/article/10.1186/s12967-019-02193-y#citeas |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | ICVS - Artigos em revistas internacionais / Papers in international journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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10.1186_s12967-019-02193-y.pdf | 1,35 MB | Adobe PDF | Ver/Abrir |