Utilize este identificador para referenciar este registo: https://hdl.handle.net/1822/63346

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dc.contributor.authorde Souza, Wagner Rpor
dc.contributor.authorMorais, Enyara Rezendepor
dc.contributor.authorKrohn, Nadia Gracielepor
dc.contributor.authorSavoldi, Marcelapor
dc.contributor.authorGoldman, Maria Helena Spor
dc.contributor.authorRodrigues, Fernandopor
dc.contributor.authorCaldana, Camilapor
dc.contributor.authorSemelka, Charles Tpor
dc.contributor.authorTikunov, Andrey Ppor
dc.contributor.authorMacdonald, Jeffrey Mpor
dc.contributor.authorGoldman, Gustavo Henriquepor
dc.date.accessioned2020-01-21T15:09:30Z-
dc.date.available2020-01-21T15:09:30Z-
dc.date.issued2013-
dc.identifier.citationde Souza WR, Morais ER, Krohn NG, Savoldi M, Goldman MHS, Rodrigues F, et al. (2013) Identification of Metabolic Pathways Influenced by the G-Protein Coupled Receptors GprB and GprD in Aspergillus nidulans. PLoS ONE 8(5): e62088. https://doi.org/10.1371/journal.pone.0062088por
dc.identifier.issn1932-6203-
dc.identifier.urihttps://hdl.handle.net/1822/63346-
dc.description.abstractHeterotrimeric G-protein-mediated signaling pathways play a pivotal role in transmembrane signaling in eukaryotes. Our main aim was to identify signaling pathways regulated by A. nidulans GprB and GprD G-protein coupled receptors (GPCRs). When these two null mutant strains were compared to the wild-type strain, the ΔgprB mutant showed an increased protein kinase A (PKA) activity while growing in glucose 1% and during starvation. In contrast, the ΔgprD has a much lower PKA activity upon starvation. Transcriptomics and (1)H NMR-based metabolomics were performed on two single null mutants grown on glucose. We noted modulation in the expression of 11 secondary metabolism gene clusters when the ΔgprB and ΔgprD mutant strains were grown in 1% glucose. Several members of the sterigmatocystin-aflatoxin gene cluster presented down-regulation in both mutant strains. The genes of the NR-PKS monodictyphenone biosynthesis cluster had overall increased mRNA accumulation in ΔgprB, while in the ΔgprD mutant strain the genes had decreased mRNA accumulation. Principal component analysis of the metabolomic data demonstrated that there was a significant metabolite shift in the ΔgprD strain. The (1)H NMR analysis revealed significant expression of essential amino acids with elevated levels in the ΔgprD strain, compared to the wild-type and ΔgprB strains. With the results, we demonstrated the differential expression of a variety of genes related mainly to secondary metabolism, sexual development, stress signaling, and amino acid metabolism. We propose that the absence of GPCRs triggered stress responses at the genetic level. The data suggested an intimate relationship among different G-protein coupled receptors, fine-tune regulation of secondary and amino acid metabolisms, and fungal development.por
dc.description.sponsorshipThe authors would like to thank FAPESP (Fundacao para Amparo a Pesquisa do Estado de Sao Paulo) and CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico) for financial support. Funding for the metabolomic studies was provided by NIH grants P30ES10126. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.por
dc.language.isoengpor
dc.publisherPublic Library of Science (PLOS)por
dc.rightsopenAccesspor
dc.subjectAspergillus nidulanspor
dc.subjectCarbohydrate Metabolismpor
dc.subjectCulture Mediapor
dc.subjectCyclic AMP-Dependent Protein Kinasespor
dc.subjectFungal Proteinspor
dc.subjectGene Expression Regulation, Fungalpor
dc.subjectGene Knockout Techniquespor
dc.subjectGlucosepor
dc.subjectHeterotrimeric GTP-Binding Proteinspor
dc.subjectMetabolomepor
dc.subjectMultigene Familypor
dc.subjectPhenotypepor
dc.subjectProtein Transportpor
dc.subjectReceptors, G-Protein-Coupledpor
dc.subjectSignal Transductionpor
dc.subjectTranscriptomepor
dc.subjectMetabolic Networks and Pathwayspor
dc.titleIdentification of metabolic pathways influenced by the G-protein coupled receptors GprB and GprD in Aspergillus nidulanspor
dc.typearticlepor
dc.peerreviewedyespor
dc.relation.publisherversionhttps://journals.plos.org/plosone/article/authors?id=10.1371/journal.pone.0062088por
oaire.citationIssue5por
oaire.citationVolume8por
dc.identifier.doi10.1371/journal.pone.0062088por
dc.identifier.pmid23658706por
dc.subject.wosScience & Technologypor
sdum.journalPLoS ONEpor
oaire.versionVoRpor
Aparece nas coleções:ICVS - Artigos em revistas internacionais / Papers in international journals

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