Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/62304
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Campo DC | Valor | Idioma |
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dc.contributor.author | Nora, Luísa Czamanski | por |
dc.contributor.author | Gonçales, Relber Aguiar | por |
dc.contributor.author | Martins-Santana, Leonardo | por |
dc.contributor.author | Ferreira, Beatriz Henriques | por |
dc.contributor.author | Rodrigues, Fernando José dos Santos | por |
dc.contributor.author | Silva-Rocha, Rafael | por |
dc.date.accessioned | 2019-11-21T13:24:34Z | - |
dc.date.available | 2019-11-21T13:24:34Z | - |
dc.date.issued | 2019 | - |
dc.identifier.issn | 1415-4757 | - |
dc.identifier.uri | https://hdl.handle.net/1822/62304 | - |
dc.description.abstract | We present a collection of minimalist binary vectors for transformation through ATMT applicable to several fungi species. pLUO plasmid binary vectors consist of a reporter module containing fluorescent proteins, mCherry or eGFP, flanked by a multiple cloning site and a transcription terminator site. They also present a synthetic gene allowing resistance to Hygromicin B flanked by alternate promoters, one for yeast and another for filamentous fungi. Left and right borders were added for Agrobacterium tumefaciens recognition, and a minimal broad-host range RK2 replication origin. Transformation was validated in the pathogenic fungus Paracoccidioides lutzii. Hence, we developed an efficient and reliable molecular tool for fungal transformation: minimalist, synthetic, modular, and available in four different versions, and these can still be readily modified using a few primers and few cloning steps. | por |
dc.description.sponsorship | LCN, LMS, RAG and RSR were supported by FAPESP (Project numbers: 2016/03763-3, 2016/01946-3, 2014/22561-7 and 2012/22921-8). FR and BF were supported by the Northern Portugal Regional Operational Programme (NORTE 2020), under the Portugal 2020 Partnership Agreement, through the European Regional Development Fund (FEDER) (NORTE-01-0145-FEDER000013). | por |
dc.language.iso | eng | por |
dc.publisher | Sociedade Brasileira de Genética | por |
dc.rights | openAccess | por |
dc.subject | Synthetic biology | por |
dc.subject | Fungi | por |
dc.subject | Agrobacterium tumefaciens | por |
dc.subject | Transformation | por |
dc.subject | Vectors | por |
dc.title | Synthetic and minimalist vectors for Agrobacterium tumefaciens-mediated transformation of fungi | por |
dc.type | article | por |
dc.peerreviewed | yes | por |
oaire.citationStartPage | 395 | por |
oaire.citationEndPage | 398 | por |
oaire.citationIssue | 2 | por |
oaire.citationVolume | 42 | por |
dc.identifier.eissn | 1678-4685 | - |
dc.identifier.doi | 10.1590/1678-4685-GMB-2018-0221 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Genetics and Molecular Biology | por |
Aparece nas coleções: | ICVS - Artigos em revistas internacionais / Papers in international journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Nora LC et al. Genet Mol Biol.pdf | 1,07 MB | Adobe PDF | Ver/Abrir |