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TitleQuantification of trans-resveratrol-loaded solid lipid nanoparticles by a validated reverse-phase HPLC photodiode array
Author(s)Rigon, Roberta B.
Fachinetti, Naiara
Severino, Patrí­cia
Durazzo, Alessandra
Lucarini, Massimo
Atanasov, Atanas G.
El Mamouni, Soukaina
Chorilli, Marlus
Santini, Antonello
Souto, Eliana
solid lipid nanoparticles
high-performance liquid chromatography
Issue date2019
JournalApplied Sciences
CitationRigon, Roberta B.; Fachinetti, Naiara; Severino, Patrí­cia; Durazzo, Alessandra; Lucarini, Massimo; Atanasov, Atanas G.; El Mamouni, Soukaina; Chorilli, Marlus; Santini, Antonello; Souto, Eliana, Quantification of trans-resveratrol-loaded solid lipid nanoparticles by a validated reverse-phase HPLC photodiode array. Applied Sciences-Basel, 9(22), 4961, 2019
Abstract(s)A new method based on reverse-phase HPLC combined with photodiode array (PDA) was developed to quantify the release of trans-resveratrol (tRES) from solid lipid nanoparticles (SLN). The mobile phase was composed of 75:0:25 (V/V) water/methanol/acetonitrile at 03.5 min, 32.5:30.0:37.5 (V/V) water/methanol/acetonitrile at 3.65.8 min, and 75:0:25 (V/V) water/methanol/acetonitrile at 5.910 min. The flow rate was set at 1.0mL/min, and tRES was detected at the wavelength of 306.6 nm. A concentration range of 1100 µg/mL was used to obtain the linear calibration curve. SLN were produced by ultrasound technique to load 0.1% (wt/wt) of tRES, and the in vitro release of the drug was run in modified Franz diffusion cells. The mean recovery of tRES was found to be 96.84 ± 0.32%. The intra-assay and inter-assay coefficients of variation were less than 5%. The proposed method was applied to in vitro permeability studies, and the Weibull model was found to be the one that best fits the tRES release, which is characterized by a simultaneous lipid chain relaxation and erosion during drug release.
Publisher version
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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