Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/62227
Título: | Quantification of trans-resveratrol-loaded solid lipid nanoparticles by a validated reverse-phase HPLC photodiode array |
Autor(es): | Rigon, Roberta B. Fachinetti, Naiara Severino, Patrícia Durazzo, Alessandra Lucarini, Massimo Atanasov, Atanas G. El Mamouni, Soukaina Chorilli, Marlus Santini, Antonello Souto, Eliana B. |
Palavras-chave: | trans-resveratrol nanotechnology solid lipid nanoparticles high-performance liquid chromatography |
Data: | 2019 |
Editora: | MDPI |
Revista: | Applied Sciences |
Citação: | Rigon, Roberta B.; Fachinetti, Naiara; Severino, Patrícia; Durazzo, Alessandra; Lucarini, Massimo; Atanasov, Atanas G.; El Mamouni, Soukaina; Chorilli, Marlus; Santini, Antonello; Souto, Eliana, Quantification of trans-resveratrol-loaded solid lipid nanoparticles by a validated reverse-phase HPLC photodiode array. Applied Sciences-Basel, 9(22), 4961, 2019 |
Resumo(s): | A new method based on reverse-phase HPLC combined with photodiode array (PDA) was developed to quantify the release of trans-resveratrol (tRES) from solid lipid nanoparticles (SLN). The mobile phase was composed of 75:0:25 (V/V) water/methanol/acetonitrile at 03.5 min, 32.5:30.0:37.5 (V/V) water/methanol/acetonitrile at 3.65.8 min, and 75:0:25 (V/V) water/methanol/acetonitrile at 5.910 min. The flow rate was set at 1.0mL/min, and tRES was detected at the wavelength of 306.6 nm. A concentration range of 1100 µg/mL was used to obtain the linear calibration curve. SLN were produced by ultrasound technique to load 0.1% (wt/wt) of tRES, and the in vitro release of the drug was run in modified Franz diffusion cells. The mean recovery of tRES was found to be 96.84 ± 0.32%. The intra-assay and inter-assay coefficients of variation were less than 5%. The proposed method was applied to in vitro permeability studies, and the Weibull model was found to be the one that best fits the tRES release, which is characterized by a simultaneous lipid chain relaxation and erosion during drug release. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/62227 |
DOI: | 10.3390/app9224961 |
ISSN: | 20763417 |
e-ISSN: | 2076-3417 |
Versão da editora: | http://www.mdpi.com/journal/applsci |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_52198_1.pdf | 1,18 MB | Adobe PDF | Ver/Abrir |
Este trabalho está licenciado sob uma Licença Creative Commons