Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/62227
Registo completo
Campo DC | Valor | Idioma |
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dc.contributor.author | Rigon, Roberta B. | por |
dc.contributor.author | Fachinetti, Naiara | por |
dc.contributor.author | Severino, Patrícia | por |
dc.contributor.author | Durazzo, Alessandra | por |
dc.contributor.author | Lucarini, Massimo | por |
dc.contributor.author | Atanasov, Atanas G. | por |
dc.contributor.author | El Mamouni, Soukaina | por |
dc.contributor.author | Chorilli, Marlus | por |
dc.contributor.author | Santini, Antonello | por |
dc.contributor.author | Souto, Eliana B. | por |
dc.date.accessioned | 2019-11-19T14:00:01Z | - |
dc.date.available | 2019-11-19T14:00:01Z | - |
dc.date.issued | 2019 | - |
dc.identifier.citation | Rigon, Roberta B.; Fachinetti, Naiara; Severino, Patrícia; Durazzo, Alessandra; Lucarini, Massimo; Atanasov, Atanas G.; El Mamouni, Soukaina; Chorilli, Marlus; Santini, Antonello; Souto, Eliana, Quantification of trans-resveratrol-loaded solid lipid nanoparticles by a validated reverse-phase HPLC photodiode array. Applied Sciences-Basel, 9(22), 4961, 2019 | por |
dc.identifier.issn | 20763417 | por |
dc.identifier.uri | https://hdl.handle.net/1822/62227 | - |
dc.description.abstract | A new method based on reverse-phase HPLC combined with photodiode array (PDA) was developed to quantify the release of trans-resveratrol (tRES) from solid lipid nanoparticles (SLN). The mobile phase was composed of 75:0:25 (V/V) water/methanol/acetonitrile at 03.5 min, 32.5:30.0:37.5 (V/V) water/methanol/acetonitrile at 3.65.8 min, and 75:0:25 (V/V) water/methanol/acetonitrile at 5.910 min. The flow rate was set at 1.0mL/min, and tRES was detected at the wavelength of 306.6 nm. A concentration range of 1100 µg/mL was used to obtain the linear calibration curve. SLN were produced by ultrasound technique to load 0.1% (wt/wt) of tRES, and the in vitro release of the drug was run in modified Franz diffusion cells. The mean recovery of tRES was found to be 96.84 ± 0.32%. The intra-assay and inter-assay coefficients of variation were less than 5%. The proposed method was applied to in vitro permeability studies, and the Weibull model was found to be the one that best fits the tRES release, which is characterized by a simultaneous lipid chain relaxation and erosion during drug release. | por |
dc.description.sponsorship | The authors wish to acknowledge the financial support from CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior) and FAPESP (Fundação de Amparo à Pesquisa do Estado de São Paulo). The financial support was also received from The Portuguese Science and Technology Foundation (FCT/MCT) and from European Funds (PRODER/COMPETE) under the project M-ERA-NET-0004/2015-PAIRED, co-financed by FEDER, under the Partnership Agreement PT2020. Authors also thank the support of the project: Nutraceutica come supporto nutrizionale nel paziente oncologico; CUP: B83D18000140007. | por |
dc.language.iso | eng | por |
dc.publisher | MDPI | por |
dc.rights | openAccess | por |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | por |
dc.subject | trans-resveratrol | por |
dc.subject | nanotechnology | por |
dc.subject | solid lipid nanoparticles | por |
dc.subject | high-performance liquid chromatography | por |
dc.title | Quantification of trans-resveratrol-loaded solid lipid nanoparticles by a validated reverse-phase HPLC photodiode array | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://www.mdpi.com/journal/applsci | por |
dc.comments | CEB52198 | por |
oaire.citationIssue | 22 | por |
oaire.citationVolume | 9 | por |
dc.date.updated | 2019-11-19T13:29:13Z | - |
dc.identifier.eissn | 2076-3417 | por |
dc.identifier.doi | 10.3390/app9224961 | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | - |
dc.subject.wos | Science & Technology | por |
sdum.journal | Applied Sciences | por |
oaire.version | VoR | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_52198_1.pdf | 1,18 MB | Adobe PDF | Ver/Abrir |
Este trabalho está licenciado sob uma Licença Creative Commons