Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/61999
Título: | iNKT cell production of GM-CSF controls Mycobacterium tuberculosis |
Autor(es): | Rothchild, Alissa C. Jayaraman, Pushpa Alves, Cláudio Behar, Samuel M. |
Palavras-chave: | Animals Granulocyte-Macrophage Colony-Stimulating Factor Interferon-gamma Interleukin-12 Interleukin-18 Macrophages, Peritoneal Mice Mice, Knockout Mycobacterium tuberculosis Natural Killer T-Cells Tuberculosis Lymphocyte Activation |
Data: | Jan-2014 |
Editora: | Public Library of Science (PLOS) |
Revista: | PLoS Pathogens |
Citação: | Rothchild, A. C., Jayaraman, P., Nunes-Alves, C., & Behar, S. M. (2014). iNKT cell production of GM-CSF controls Mycobacterium tuberculosis. PLoS pathogens, 10(1), e1003805. |
Resumo(s): | Invariant natural killer T (iNKT) cells are activated during infection, but how they limit microbial growth is unknown in most cases. We investigated how iNKT cells suppress intracellular Mycobacterium tuberculosis (Mtb) replication. When co-cultured with infected macrophages, iNKT cell activation, as measured by CD25 upregulation and IFNγ production, was primarily driven by IL-12 and IL-18. In contrast, iNKT cell control of Mtb growth was CD1d-dependent, and did not require IL-12, IL-18, or IFNγ. This demonstrated that conventional activation markers did not correlate with iNKT cell effector function during Mtb infection. iNKT cell control of Mtb replication was also independent of TNF and cell-mediated cytotoxicity. By dissociating cytokine-driven activation and CD1d-restricted effector function, we uncovered a novel mediator of iNKT cell antimicrobial activity: GM-CSF. iNKT cells produced GM-CSF in vitro and in vivo in a CD1d-dependent manner during Mtb infection, and GM-CSF was both necessary and sufficient to control Mtb growth. Here, we have identified GM-CSF production as a novel iNKT cell antimicrobial effector function and uncovered a potential role for GM-CSF in T cell immunity against Mtb. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/61999 |
DOI: | 10.1371/journal.ppat.1003805 |
ISSN: | 1553-7366 |
e-ISSN: | 1553-7374 |
Versão da editora: | https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1003805 |
Arbitragem científica: | yes |
Acesso: | Acesso restrito UMinho |
Aparece nas coleções: | ICVS - Artigos em revistas internacionais / Papers in international journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Rothchild-2014-Inkt-cell-production-of-gm-csf-cont.pdf Acesso restrito! | 986,03 kB | Adobe PDF | Ver/Abrir |