Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/58941
Registo completo
Campo DC | Valor | Idioma |
---|---|---|
dc.contributor.author | Reis-Filho, Jorge S. | por |
dc.contributor.author | Preto, Ana | por |
dc.contributor.author | Soares, Paula | por |
dc.contributor.author | Ricardo, Sara | por |
dc.contributor.author | Cameselle-Teijeiro, José | por |
dc.contributor.author | Sobrinho-Simões, Manuel | por |
dc.date.accessioned | 2019-02-06T12:29:30Z | - |
dc.date.issued | 2003-01 | - |
dc.identifier.issn | 0893-3952 | por |
dc.identifier.uri | https://hdl.handle.net/1822/58941 | - |
dc.description.abstract | Solid cell nests of the thyroid are embryonic remnants of endodermal origin that may be difficult to distinguish from squamous metaplasia, metastatic squamous carcinoma, papillary microcarcinoma, medullary carcinoma, and C-cell hyperplasia. These embryonic structures are composed of main cells and C-cells; cystic structures and mixed follicles are sometimes observed intermingled with solid cell nests. Recently, p63, a p53 homologue that is consistently expressed in basal/stem cells of stratified epithelia and plays a major role in triggering the differentiation of some specific cell lineages, has been characterized. We evaluated the immunohistochemical expression of p63, cytokeratins (CAM 5.2, AE1/AE3, 34betaE12, 7, and 20), carcinoembryonic antigen, thyroid transcription factor 1 (TTF-1), thyroglobulin, and calcitonin using the streptavidin-biotin-peroxidase complex technique in 6 bona fide solid cell nests. We observed that main cells of solid cell nests are strongly decorated by p63, while C-cells and all other thyroid structures were consistently negative. Moreover, main cells expressed carcinoembryonic antigen and all cytokeratins but cytokeratin 20 and lacked TTF-1, thyroglobulin and calcitonin. In contrast to this, C-cells of solid cell nests were immunoreactive for calcitonin, CAM 5.2, AE1/AE3, and cytokeratin 7; focal immunoreactivity for TTF-1 was also observed in some C-cells. We conclude that main cells of the solid cell nests display a basal/stem cell phenotype (p63 and basal cytokeratin positivity), whereas C-cells show features of parafollicular differentiation. We conclude, furthermore, that p63 antibodies may help in distinguishing solid cell nests from their mimics. | por |
dc.description.sponsorship | This study was partially supported by Ph.D. Grants from the Portuguese Science and Technology Foundation (FCT) (references PRAXIS XXI/BD/21795/99 - AP and SFRH/BD/5386/2001 - JSRF) and Programa Operacional Ciência, Tecnologia e Inovação (POCTI) do Quadro Comunitário de Apoio (QCA) II. | por |
dc.language.iso | eng | por |
dc.publisher | Nature Publishing Group | por |
dc.relation | SFRH/BD/5386/2001 | por |
dc.rights | restrictedAccess | por |
dc.subject | Adenoma | por |
dc.subject | Adult | por |
dc.subject | Aged | por |
dc.subject | Biomarkers, Tumor | por |
dc.subject | Carcinoembryonic Antigen | por |
dc.subject | Carcinoma, Papillary | por |
dc.subject | DNA-Binding Proteins | por |
dc.subject | Female | por |
dc.subject | Genes, Tumor Suppressor | por |
dc.subject | Humans | por |
dc.subject | Immunoenzyme Techniques | por |
dc.subject | Keratins | por |
dc.subject | Male | por |
dc.subject | Middle Aged | por |
dc.subject | Phosphoproteins | por |
dc.subject | Stem Cells | por |
dc.subject | Thyroid Neoplasms | por |
dc.subject | Trans-Activators | por |
dc.subject | Transcription Factors | por |
dc.subject | Tumor Suppressor Proteins | por |
dc.subject | Membrane Proteins | por |
dc.subject | p63 | por |
dc.subject | immunohistochemistry | por |
dc.subject | thyroid | por |
dc.subject | ultimobranchial body | por |
dc.title | p63 expression in solid cell nests of the thyroid: further evidence for a stem cell origin | por |
dc.type | article | por |
dc.peerreviewed | yes | por |
oaire.citationStartPage | 43 | por |
oaire.citationEndPage | 48 | por |
oaire.citationIssue | 1 | por |
oaire.citationVolume | 16 | por |
dc.identifier.doi | 10.1097/01.MP.0000047306.72278.39 | por |
dc.identifier.pmid | 12527712 | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Modern Pathology | por |
Aparece nas coleções: | ICVS - Artigos em revistas internacionais / Papers in international journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
---|---|---|---|---|
3880708.pdf Acesso restrito! | 810,51 kB | Adobe PDF | Ver/Abrir |