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https://hdl.handle.net/1822/58255
Título: | Methylation of the hsa-miR-124, SOX1, TERT, and LMX1A genes as biomarkers for precursor lesions in cervical cancer |
Autor(es): | Rogeri, Caroline Domingues Silveira, Henrique César Santejo Causin, Rhafaela Lima Villa, Luisa Lina Stein, Maíra Degiovani de Carvalho, Ana Carolina Arantes, Lídia Maria Rebolho Batista Scapulatempo-Neto, Cristovam Possati-Resende, Júlio César Antoniazzi, Márcio Longatto, Adhemar Fregnani, José Humberto Tavares Guerreiro |
Palavras-chave: | Adult Biomarkers, Tumor Cervical Intraepithelial Neoplasia Early Detection of Cancer Female Humans LIM-Homeodomain Proteins MicroRNAs Middle Aged Papillomaviridae Papillomavirus Infections Promoter Regions, Genetic SOXB1 Transcription Factors Sensitivity and Specificity Telomerase Transcription Factors Uterine Cervical Neoplasms DNA Methylation Cervical cancer prevention Cervical cytology Human papillomavirus Tumour suppressor genes |
Data: | Set-2018 |
Editora: | Elsevier 1 |
Revista: | Gynecologic Oncology |
Citação: | Rogeri, C. D., Silveira, H. C. S., Causin, R. L., Villa, L. L., Stein, M. D., de Carvalho, A. C., ... & Longatto-Filho, A. (2018). Methylation of the hsa-miR-124, SOX1, TERT, and LMX1A genes as biomarkers for precursor lesions in cervical cancer. Gynecologic oncology, 150(3), 545-551 |
Resumo(s): | The methylation profile of genes in precursor lesions in cervical cancer was characterized to improve screening techniques for high-grade intraepithelial neoplasia. Objectives The methylation profile of genes in precursor lesions in cervical cancer was characterized to improve screening techniques for high-grade intraepithelial neoplasia. Methods A total of 447 cervical cytology samples obtained from women who underwent colposcopy were examined. The cases were distributed as follows: (1) cervices without cervical intraepithelial neoplasia (CIN; n = 152); (2) cervices with a CIN grade of 1 (CIN 1; n = 147); and (3) cervices with a CIN grade of 2 or 3 (CIN 2/3; n = 148). The methylation pattern for a panel of 15 genes was analysed by quantitative methylation-specific PCR (qMSP) and compared between the groups (≤CIN 1 vs. CIN 2+). Results In the validation set, seven genes presented significantly different methylation profiles according to diagnosis, namely, DAPK1 (p = 0.001), EPB41L3 (p = 0.001), HIC1 (p = 0.028), hsa-miR-124-2 (p = 0.001), LMX1A (p = 0.001), SOX1 (p = 0.001), and TERT (p = 0.001). Six genes showed a significant increase in the frequency of methylation in the presence of hr-HPV, namely, DAPK1 (p = 0.001), EPB41L3 (p = 0.001), hsa-miR-124-2 (p = 0.001), LMX1A (p = 0.001), SOX1 (p = 0.001), and TERT (p = 0.001). The methylation of the hsa-miR-124 gene showed sensitivity and specificity (86.7% and 61.3%, respectively) similar to that of the HPV test (91.3% and 50.0%, respectively). The independent factors associated with the diagnosis of CIN 2+ and the methylation of the hsa-miR-124-2 (OR = 5.1), SOX1 (OR = 2.8), TERT (OR = 2.2), and LMX1A (OR = 2.0) genes were a positive test for hr-HPV (odds ratio [OR] = 5.5). Conclusions Hypermethylation of the hsa-miR-124-2, SOX1, TERT, and LMX1A genes may be a promising biomarker for precursor lesions in cervical cancer regardless of the hr-HPV status. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/58255 |
DOI: | 10.1016/j.ygyno.2018.06.014 |
ISSN: | 0090-8258 |
e-ISSN: | 1095-6859 |
Versão da editora: | https://www.sciencedirect.com/science/article/pii/S0090825818309909 |
Arbitragem científica: | yes |
Acesso: | Acesso restrito UMinho |
Aparece nas coleções: | ICVS - Artigos em revistas internacionais / Papers in international journals |
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rogeri.pdf Acesso restrito! | 360,23 kB | Adobe PDF | Ver/Abrir |