Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/58252

TitleDetection of sputum cofilin-1 as indicator of malignancy
Author(s)Rangel, M. P.
Antonangelo, L.
Acencio, M. M. P.
Faria, C. S.
de Sá, V. K.
Leão, P. S.
Farhat, C.
Fabro, A T
Longatto, Adhemar
Reis, R. M.
Takagaki, T.
Capelozzi, V. L.
KeywordsAdult
Aged
Biomarkers, Tumor
Carcinoma, Squamous Cell
Case-Control Studies
Cell Proliferation
Cofilin 1
Enzyme-Linked Immunosorbent Assay
Female
Humans
Lung Neoplasms
Male
Middle Aged
Neoplasm Invasiveness
Neoplasm Staging
Prognosis
ROC Curve
Sensitivity and Specificity
Sputum
Lung cancer
Liquid-biopsy
Metastases
Biomarker
Issue date2018
PublisherAssociação Brasileira de Divulgação Científica (ABRADIC)
JournalBrazilian Journal of Medical and Biological Research
CitationRangel, M. P., Antonangelo, L., Acencio, M. M. P., Faria, C. S., de Sá, V. K., Leão, P. S., ... & Takagaki, T. (2018). Detection of sputum cofilin-1 as indicator of malignancy. Brazilian Journal of Medical and Biological Research, 51(8)
Abstract(s)Cofilin-1 (CFL1), a small protein of 18 kDa, has been studied as a biomarker due to its involvement in tumor cell migration and invasion. Our aim was to evaluate CFL1 as an indicator of malignancy and aggressiveness in sputum samples. CFL1 was analyzed by ELISA immunoassay in the sputum of 73 lung cancer patients, 13 cancer-free patients, and 6 healthy volunteers. Statistical analyses included ANOVA, ROC curves, Spearman correlation, and logistic regression. Sputum CFL1 levels were increased in cancer patients compared to cancer-free patients and volunteers (P<0.05). High expression of sputum CFL1 was correlated to T4 stage (P=0.01) and N stage (P=0.03), tobacco history (P=0.01), and squamous cell carcinoma histologic type (P=0.04). The accuracy of sputum CFL1 in discriminating cancer patients from cancer-free patients and healthy volunteers were 0.78 and 0.69, respectively. CFL1 at a cut-off value of 415.25 pg/mL showed sensitivity/specificity of 0.80/0.70 in differentiating between healthy volunteers and cancer patients. Sputum CFL1 was also able to identify cancer-free patients from patients with lung cancer. The AUC was 0.70 and, at a cut-off point ≥662.63 pg/mL, we obtained 60% sensitivity and 54% specificity. Logistic regression analysis controlled for tobacco history, histologic types, and N stage showed that cancer cell-associated CFL1 was an independent predictor of death. Smoker patients with squamous cell carcinoma, lymph node metastasis and sputum CFL1>1.475 pg/mL showed augmented chance of death, suggesting lung cancer aggressiveness. CFL1 presented diagnostic value in detecting lung cancer and was associated to tumor aggressiveness.
TypeArticle
URIhttp://hdl.handle.net/1822/58252
DOI10.1590/1414-431x20187138
ISSN0100-879X
e-ISSN1414-431X
Publisher versionhttp://www.scielo.br/scielo.php?pid=S0100-879X2018000800603&script=sci_arttext
Peer-Reviewedyes
AccessOpen access
Appears in Collections:ICVS - Artigos em Revistas Internacionais com Referee

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