Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/58194
Título: | Construction and characterization of a new TRAIL soluble form, active at picomolar concentrations |
Autor(es): | Melendez, Matias Eliseo Silva-Oliveira, Renato José Silva Almeida Vicente, Anna Luiza Rebolho Batista Arantes, Lidia Maria Carolina de Carvalho, Ana Epstein, Alberto Luis Reis, R. M. Carvalho, André Lopes |
Palavras-chave: | TRAIL Apoptosis Cancer treatment Amplicon vectors |
Data: | 5-Jun-2018 |
Editora: | Impact Journals |
Revista: | Oncotarget |
Citação: | Melendez, M. E., Silva-Oliveira, R. J., Vicente, A. L. S. A., Arantes, L. M. R. B., de Carvalho, A. C., Epstein, A. L., ... & Carvalho, A. L. (2018). Construction and characterization of a new TRAIL soluble form, active at picomolar concentrations. Oncotarget, 9(43), 27233. |
Resumo(s): | Apoptosis induction has emerged as a treatment option for anticancer therapy. Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), a type II transmembrane protein, is a potent and specific pro-apoptotic protein ligand, which activates the extrinsic apoptosis pathway of the cell death receptors. Here we describe the construction and characterization of a new soluble TRAIL, sfTRAIL, stabilized with the trimerization Foldon domain from the Fibritin protein of the bacteriophage T4. Supernatants of 0.22 μM-filtered supernatants were produced in Vero-transduced cells with HSV1-derived viral amplicon vectors. Experiments were undertaken in two known TRAIL-sensitive (U373 and MDA.MB.231) and two TRAIL-resistant (MCF7 and A549) cell lines, to determine (i) whether the sfTRAIL protein is synthetized and, (ii) whether sfTRAIL could induce receptor-mediated apoptosis. Our results showed that sfTRAIL was able to induce apoptosis at concentrations as low as 1899.29 pg/mL (27.71 pM), independently of caspase-9 activation, and reduction in cell viability at 998.73 fM. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/58194 |
DOI: | 10.18632/oncotarget.25519 |
e-ISSN: | 1949-2553 |
Versão da editora: | http://www.oncotarget.com/index.php?journal=oncotarget&page=article&op=view&path[]=25519&path[]=79903 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | ICVS - Artigos em revistas internacionais / Papers in international journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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melendez 2018.pdf | 2,6 MB | Adobe PDF | Ver/Abrir |