Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/57992

TitleAdrenoceptor-stimulated inflammatory response in stress-induced serum amyloid A synthesis
Author(s)Konstandi, Maria
Sotiropoulos, I.
Matsubara, Tsutomu
Malliou, Foteini
Katsogridaki, Alexandra
Andriopoulou, Christina E.
Gonzalez, Frank J.
KeywordsAdrenoceptors
IL-1β
IL-6
SAA1/2
SAA3
Stress
TNFα
SAA1
2
IL-1 beta
Issue date6-Jan-2019
PublisherSpringer-Verlag
JournalPsychopharmacology
Abstract(s)Rationale Stressful life events are suggested to contribute to the development of various pathologies, such as cardiovascular disorders, whose etiopathogenesis is highly associated with elevated levels of serum amyloid A (SAA) proteins. SAA synthesis inthe liver isregulated bya complex network ofcytokines actingindependently orinconcert withvarious hormones/stimulants including the stress-activated sympathetic nervous system. Objective This study aims to investigate the underlying mechanisms that regulate the stress-induced hepatic synthesis of SAA, with particular focus on adrenoceptors (AR), major components of the sympathoadrenal response to stress. Methods and results We demonstrated that repeated stress elevates IL-1β, IL-6, and TNFα serum levels in mice, accompanied by increased synthesis and secretion of hepatic SAA1/2 and SAA3, an effect that was blocked by AR antagonists. Moreover, stimulation ofα1- andβ1/2-ARsmimics thestress effectonSAA1/2 regulation, whereas α2-AR stimulation exhibitsa relatively weakimpactonSAA.InsupportoftheessentialcytokinecontributionintheAR-agonistinducedSAAproductionisthefactthat theanti-inflammatorydrug,sodiumsalicylate,preventedtheAR-stimulatedhepaticSAA1/2synthesisbyreducingIL-1βlevels, whereasIL-1βinhibitionwithAnakinramimicsthissodiumsalicylatepreventiveeffect,thusindicatingacrucial rolefor IL-1β. Interestingly, the AR-driven SAA3 synthesis was elevated by sodium salicylate in a TNFα-dependent way, supporting diverse and complex regulatory roles of cytokines in SAA production. In contrast to α1/α2-AR, the β1/2-AR-mediated SAA1/2 and SAA3 upregulation cannot be reversed by fenofibrate, a hypolipidemic drug with anti-inflammatory properties. Conclusion Taken together, these findings strongly support a critical role of the AR-stimulated inflammatory response in the hepatic SAA production under stressful conditions, highlighting distinct AR type-specific mechanisms that regulate the hepatic synthesis of SAA1/2 and SAA3.
TypePreprint
URIhttp://hdl.handle.net/1822/57992
DOI10.1007/s00213-018-5149-4
ISSN0033-3158
e-ISSN1432-2072
Publisher versionhttps://link.springer.com/content/pdf/10.1007/s00213-018-5149-4.pdf
Peer-Reviewedyes
AccessEmbargoed access (1 Year)
Appears in Collections:ICVS - Artigos em Revistas Internacionais com Referee

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