Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/57954

TitleSorting nexin 3 mutation impairs development and neuronal function in Caenorhabditis elegans
Author(s)Vieira, Neide
Bessa, Carlos
Rodrigues, Ana J.
Marques, Paulo
Chan, Fung-Yi
Carvalho, Ana Xavier de
Neves, Margarida Correia
Sousa, Nuno
KeywordsBehavior
Neuronal defects
Impaired development
Nervous system
Sorting nexins
Issue dateJun-2018
PublisherSpringer
JournalCellular and Molecular Life Sciences
Abstract(s)The sorting nexins family of proteins (SNXs) plays pleiotropic functions in protein trafficking and intracellular signaling and has been associated with several disorders, namely Alzheimer's disease and Down's syndrome. Despite the growing association of SNXs with neurodegeneration, not much is known about their function in the nervous system. The aim of this work was to use the nematode Caenorhabditis elegans that encodes in its genome eight SNXs orthologs, to dissect the role of distinct SNXs, particularly in the nervous system. By screening the C. elegans SNXs deletion mutants for morphological, developmental and behavioral alterations, we show here that snx-3 gene mutation leads to an array of developmental defects, such as delayed hatching, decreased brood size and life span and reduced body length. Additionally, ∆snx-3 worms present increased susceptibility to osmotic, thermo and oxidative stress and distinct behavioral deficits, namely, a chemotaxis defect which is independent of the described snx-3 role in Wnt secretion. ∆snx-3 animals also display abnormal GABAergic neuronal architecture and wiring and altered AIY interneuron structure. Pan-neuronal expression of C. elegans snx-3 cDNA in the ∆snx-3 mutant is able to rescue its locomotion defects, as well as its chemotaxis toward isoamyl alcohol. Altogether, the present work provides the first in vivo evidence of the SNX-3 role in the nervous system.
TypeArticle
URIhttp://hdl.handle.net/1822/57954
DOI10.1007/s00018-017-2719-2
ISSN1420-682X
e-ISSN1420-9071
Peer-Reviewedyes
AccessEmbargoed access (1 Year)
Appears in Collections:ICVS - Artigos em Revistas Internacionais com Referee

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