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https://hdl.handle.net/1822/5785
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Campo DC | Valor | Idioma |
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dc.contributor.author | Paredes, Joana | - |
dc.contributor.author | Albergaria, André | - |
dc.contributor.author | Oliveira, J. T. | - |
dc.contributor.author | Jerónimo, Carmen | - |
dc.contributor.author | Milanezi, Fernanda | - |
dc.contributor.author | Schmitt, Fernando C. | - |
dc.date.accessioned | 2006-11-15T17:21:28Z | - |
dc.date.available | 2006-11-15T17:21:28Z | - |
dc.date.issued | 2005-08 | - |
dc.identifier.citation | "Clinical cancer research". ISSN 1078-0432. 11:16 (Aug. 2005) 186-5877. | eng |
dc.identifier.issn | 1078-0432 | eng |
dc.identifier.uri | https://hdl.handle.net/1822/5785 | - |
dc.description.abstract | Purpose: P-cadherin overexpressionhas been reported in breast carcinomas,where itwas associated with proliferative high-grade histological tumors. This study aimed to analyze P-cadherin expression in invasive breast cancer and to correlate it with tumor markers, pathologic features, and patient survival. Another purpose was to evaluate the P-cadherin promoter methylation pattern as the molecularmechanismunderlying this gene regulation. Experimental Design: Using a series of invasive breast carcinomas, P-cadherin expressionwas evaluated and correlated with histologic grade, estrogen receptor, MIB-1, and p53 and c-erbB-2 expression. In order to assess whether P-cadherin expression was associated with changes in CDH3 promoter methylation, we studied the methylation status of a gene 5V-flanking region in these same carcinomas.This analysis was also done for normal tissue and for a breast cancer cell line treatedwith a demethylating agent. Results: P-cadherinexpression showeda strong correlationwithhighhistologic grade, increased proliferation, c-erbB-2 and p53 expression, lack of estrogen receptor, and poor patient survival. This overexpressioncanbe regulatedby gene promotermethylationbecause the 5-Aza-2V-deoxycytidine treatment ofMCF-7/AZ cells increased P-cadherinmRNA and proteinlevels. Additionally, we found that 71% of P- adherin-negative cases showed promoter methylation,whereas 65% of positive ones were unmethylated (P = 0.005). The normal P-cadherin-negative breast epithelial cells showed consistent CDH3 promotermethylation. Conclusions: P-cadherin expressionwas strongly associatedwith tumor aggressiveness, being a good indicator of clinical outcome. Moreover, the aberrant expression of P-cadherin in breast cancermight be regulated by gene promoter hypomethylation. | eng |
dc.language.iso | eng | eng |
dc.publisher | American Association for Cancer Research | por |
dc.rights | openAccess | eng |
dc.title | P-cadherin overexpression Is an indicator of clinical outcome in invasive breast carcinomas and is associated with CDH3 promoter hypomethylation | eng |
dc.type | article | por |
dc.peerreviewed | yes | eng |
sdum.number | 16 | eng |
sdum.pagination | 5869-5877 | eng |
sdum.publicationstatus | published | eng |
sdum.volume | 11 | eng |
oaire.citationStartPage | 5869 | por |
oaire.citationEndPage | 5877 | por |
oaire.citationIssue | 16 | por |
oaire.citationVolume | 11 | por |
dc.identifier.doi | 10.1158/1078-0432.CCR-05-0059 | por |
dc.identifier.pmid | 16115928 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Clinical cancer research | por |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals ICVS - Artigos em revistas internacionais / Papers in international journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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P-CadClinCanResea_2005.pdf | 359,65 kB | Adobe PDF | Ver/Abrir |