Utilize este identificador para referenciar este registo: http://hdl.handle.net/1822/5680

TítuloFunctional purification of the monocarboxylate transporter of the yeast Candida utilis
Autor(es)Baltazar, Fátima
Cássio, Fernanda
Leão, Cecília
Palavras-chaveCandida utilis
Functional reconstitution
Lactate carrier
Protein purification
Data2006
EditoraSpringer
RevistaBiotechnology Letters
Citação"Biotechnol lett". ISSN 0141-5492. 28:16 (Aug. 2006) 1221-1226.
Resumo(s)Plasma membranes of the yeast, Candida utilis, were solubilized with octyl-b-D- lucopyranoside and a fraction enriched in the lactate carrier was obtained with DEAE-Sepharose anion- exchange chromatography, after elution with 0.4 M NaCl. The uptake of lactic acid into proteoliposomes, containing the purified protein fraction and cytochrome c oxidase, was dependent on a proton-motive force and the transport specificity was consistent with the one of C. utilis intact cells. Overall, we have obtained a plasma membrane fraction enriched in the lactate carrier of C. utilis in which the transport properties were preserved. Given the similarities between the lactate transport of C. utilis and the one of mammalian cells, this purified system could be further explored to screen for specific lactate inhibitors, with potential therapeutic applications.
Tipoarticle
URIhttp://hdl.handle.net/1822/5680
DOI10.1007/s10529-006-9088-5
ISSN0141-5492
Versão da editora0141-5492
Arbitragem científicayes
AcessoopenAccess
Aparece nas coleções:ICVS - Artigos em Revistas Internacionais com Referee

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