Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/54271
Título: | Silk fibroin/amniotic membrane 3D bi-layered artificial skin |
Autor(es): | Gholipourmalekabadi, Mazaher Samadikuchaksaraei, Ali Seifalian, Alexander M. Urbanska, Aleksandra M. Ghanbarian, Hossein Hardy, John G. Omrani, Mir Davood Mozafari, Masoud Reis, R. L. Kundu, Subhas C. |
Palavras-chave: | scaffold skin regenerative medicine human amniotic membrane silk fibroin |
Data: | 2018 |
Editora: | IOP Publishing Ltd |
Revista: | Biomedical Materials |
Resumo(s): | Burn injuries have been reported to be an important cause of morbidity and mortality and they are still considered as unmet clinical need. Although there is a myriad of effective stem cells that have been suggested for skin regeneration, there is no one ideal scaffold. The aim of this study was to develop a three-dimensional (3D) bi-layer scaffold made of biological decellularized human amniotic membrane (AM) with viscoelastic electrospun nanofibrous silk fibroin (ESF) spun on top. The fabricated 3D bi-layer AM/ESF scaffold was submerged in ethanol to induce ss-sheet transformation as well as to get a tightly coated and inseparable bi-layer. The biomechanical and biological properties of the 3D bi-layer AM/ESF scaffold were investigated. The results indicate significantly improved mechanical properties of the AM/ESF compared with the AM alone. Both the AM and AM/ESF possess a variety of suitable adhesion cells without detectable cytotoxicity against adipose tissue-derived mesenchymal stem cells (AT-MSCs). The AT-MSCs show increased expression of two main pro-angiogenesis factors, vascular endothelial growth factor and basic fibroblast growth factor, when cultured on the AM/ESF for 7 days, when comparing with AM alone. The results suggest that the AM/ ESF scaffold with autologous AT-MSCs has excellent cell adhesion and proliferation along with production of growth factors which serves as a possible application in a clinical setting for skin regeneration. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/54271 |
DOI: | 10.1088/1748-605X/aa999b |
ISSN: | 1748-6041 |
Arbitragem científica: | yes |
Acesso: | Acesso restrito UMinho |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Gholipourmalekabadi_2018_Biomed._Mater._13_035003.pdf Acesso restrito! | 3,28 MB | Adobe PDF | Ver/Abrir |