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https://hdl.handle.net/1822/54271
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Campo DC | Valor | Idioma |
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dc.contributor.author | Gholipourmalekabadi, Mazaher | por |
dc.contributor.author | Samadikuchaksaraei, Ali | por |
dc.contributor.author | Seifalian, Alexander M. | por |
dc.contributor.author | Urbanska, Aleksandra M. | por |
dc.contributor.author | Ghanbarian, Hossein | por |
dc.contributor.author | Hardy, John G. | por |
dc.contributor.author | Omrani, Mir Davood | por |
dc.contributor.author | Mozafari, Masoud | por |
dc.contributor.author | Reis, R. L. | por |
dc.contributor.author | Kundu, Subhas C. | por |
dc.date.accessioned | 2018-04-14T09:59:07Z | - |
dc.date.issued | 2018 | - |
dc.identifier.issn | 1748-6041 | por |
dc.identifier.uri | https://hdl.handle.net/1822/54271 | - |
dc.description.abstract | Burn injuries have been reported to be an important cause of morbidity and mortality and they are still considered as unmet clinical need. Although there is a myriad of effective stem cells that have been suggested for skin regeneration, there is no one ideal scaffold. The aim of this study was to develop a three-dimensional (3D) bi-layer scaffold made of biological decellularized human amniotic membrane (AM) with viscoelastic electrospun nanofibrous silk fibroin (ESF) spun on top. The fabricated 3D bi-layer AM/ESF scaffold was submerged in ethanol to induce ss-sheet transformation as well as to get a tightly coated and inseparable bi-layer. The biomechanical and biological properties of the 3D bi-layer AM/ESF scaffold were investigated. The results indicate significantly improved mechanical properties of the AM/ESF compared with the AM alone. Both the AM and AM/ESF possess a variety of suitable adhesion cells without detectable cytotoxicity against adipose tissue-derived mesenchymal stem cells (AT-MSCs). The AT-MSCs show increased expression of two main pro-angiogenesis factors, vascular endothelial growth factor and basic fibroblast growth factor, when cultured on the AM/ESF for 7 days, when comparing with AM alone. The results suggest that the AM/ ESF scaffold with autologous AT-MSCs has excellent cell adhesion and proliferation along with production of growth factors which serves as a possible application in a clinical setting for skin regeneration. | por |
dc.description.sponsorship | We express our sincere thanks to Dr Nariman Mosaffa and Dr Mohammad Salehi for providing helpful comments on this work. SC Kundu presently holds an ERA Chair Full Professor position at the 3Bs Research Group, University of Minho, Portugal, supported by the European Union Framework Programme for Research and Innovation Horizon 2020 under grant agreement No 668983-FoReCaST. | por |
dc.language.iso | eng | por |
dc.publisher | IOP Publishing Ltd | por |
dc.relation | info:eu-repo/grantAgreement/EC/H2020/668983/EU | por |
dc.rights | restrictedAccess | por |
dc.subject | scaffold | por |
dc.subject | skin | por |
dc.subject | regenerative medicine | por |
dc.subject | human amniotic membrane | por |
dc.subject | silk fibroin | por |
dc.title | Silk fibroin/amniotic membrane 3D bi-layered artificial skin | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.comments | http://3bs.uminho.pt/node/19631 | - |
oaire.citationIssue | 3 | por |
oaire.citationVolume | 13 | por |
dc.date.updated | 2018-04-13T21:44:55Z | - |
dc.identifier.doi | 10.1088/1748-605X/aa999b | por |
dc.identifier.pmid | 29125135 | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | por |
dc.subject.wos | Science & Technology | - |
sdum.export.identifier | 5051 | - |
sdum.journal | Biomedical Materials | por |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Gholipourmalekabadi_2018_Biomed._Mater._13_035003.pdf Acesso restrito! | 3,28 MB | Adobe PDF | Ver/Abrir |