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https://hdl.handle.net/1822/50598
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Campo DC | Valor | Idioma |
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dc.contributor.author | Sousa, Nuno Eduardo Sevivas | por |
dc.contributor.author | Teixeira, Fábio Gabriel Rodrigues | por |
dc.contributor.author | Portugal, Raquel | por |
dc.contributor.author | Direito-Santos, Bruno | por |
dc.contributor.author | Espregueira-Mendes, João | por |
dc.contributor.author | Oliveira, Filipe J. | por |
dc.contributor.author | Silva, Rui F. | por |
dc.contributor.author | Sousa, Nuno | por |
dc.contributor.author | Sow, Wan Ting | por |
dc.contributor.author | Luong, Nguyen | por |
dc.contributor.author | Ng, Kee Woei | por |
dc.contributor.author | Salgado, A. J. | por |
dc.date.accessioned | 2018-02-16T14:02:06Z | - |
dc.date.issued | 2018 | - |
dc.identifier.issn | 0363-5465 | - |
dc.identifier.uri | https://hdl.handle.net/1822/50598 | - |
dc.description.abstract | BACKGROUND: Massive rotator cuff tears (MRCTs) represent a major clinical concern, especially when degeneration and chronicity are involved, which highly compromise healing capacity. PURPOSE: To study the effect of the secretome of mesenchymal stem cells (MSCs) on tendon cells (TCs) followed by the combination of these activated TCs with an electrospun keratin-based scaffold to develop a tissue engineering strategy to improve tendon-bone interface (TBi) healing in a chronic MRCT rat model. STUDY DESIGN: Controlled laboratory study. METHODS: Human TCs (hTCs) cultured with the human MSCs (hMSCs) secretome (as conditioned media [CM]) were combined with keratin electrospun scaffolds and further implanted in a chronic MRCT rat model. Wistar-Han rats (N = 15) were randomly assigned to 1 of 3 groups: untreated lesion (MRCT group, n = 5), lesion treated with a scaffold only (scaffold-only group, n = 5), and lesion treated with a scaffold seeded with hTCs preconditioned with hMSCs-CM (STC_hMSC_CM group, n = 5). After sacrifice, 16 weeks after surgery, the rotator cuff TBi was harvested for histological analysis and biomechanical testing. RESULTS: The hMSCs secretome increased hTCs viability and density in vitro. In vivo, a significant improvement of the tendon maturing score was observed in the STC_hMSC_CM group (mean ± standard error of the mean, 15.6 ± 1.08) compared with the MRCT group (11.0 ± 1.38; P < .05). Biomechanical tests revealed a significant increase in the total elongation to rupture (STC_hMSC_CM, 11.99 ± 3.30 mm; scaffold-only, 9.89 ± 3.47 mm; MRCT, 5.86 ± 3.16 mm; P < .05) as well as a lower stiffness (STC_hMSC_CM, 6.25 ± 1.74 N/mm; scaffold-only, 6.72 ± 1.28 N/mm; MRCT, 11.54 ± 2.99 N/mm; P < .01). CONCLUSION: The results demonstrated that hMSCs-CM increased hTCs viability and density in vitro. Clear benefits also were observed when these primed cells were integrated into a tissue engineering strategy with an electrospun keratin scaffold, as evidenced by improved histological and biomechanical properties for the STC_hMSC_CM group compared with the MRCT group. CLINICAL RELEVANCE: This work supports further investigation into the use of MSC secretome for priming TCs toward a more differentiated phenotype, and it promotes the tissue engineering strategy as a promising modality to help improve treatment outcomes for chronic MRCTs. | por |
dc.language.iso | eng | por |
dc.publisher | SAGE Publications | por |
dc.rights | restrictedAccess | por |
dc.subject | Massive rotator cuff tear | por |
dc.subject | hMSC secretome | por |
dc.subject | Tendon-bone healing | por |
dc.title | Mesenchymal stem cell secretome improves tendon cell viability in vitro and tendon-bone healing in vivo when a tissue engineering strategy is used in a rat model of chronic massive rotator cuff tear | por |
dc.type | article | por |
dc.peerreviewed | yes | por |
oaire.citationStartPage | 449 | por |
oaire.citationEndPage | 459 | por |
oaire.citationIssue | 2 | por |
oaire.citationVolume | 46 | por |
dc.identifier.eissn | 1552-3365 | - |
dc.identifier.doi | 10.1177/0363546517735850 | por |
dc.identifier.pmid | 29053925 | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | American Journal of Sports Medicine | por |
Aparece nas coleções: | ICVS - Artigos em revistas internacionais / Papers in international journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Mesenchymal Stem Cell Secretome Improves Tendon Cell Viability In Vitro and Tendon-Bone Healing In Vivo When a Tissue Engineering Strategy Is Used in a Rat Model of Chronic Massive Rotator Cuff Te.pdf Acesso restrito! | 4,74 MB | Adobe PDF | Ver/Abrir |