Utilize este identificador para referenciar este registo: https://hdl.handle.net/1822/50276

TítuloEvaluation of Bronchoalveolar Lavage Fluid Cytokines as Biomarkers for Invasive Pulmonary Aspergillosis in At-Risk Patients
Autor(es)Gonçalves, Samuel Martins
Lagrou, Katrien
Rodrigues, Claudia S.
Campos, Claudia F.
Bernal-Martinez, Leticia
Rodrigues, Fernando José dos Santos
Silvestre, Ricardo Jorge Leal
Alcazar-Fuoli, Laura
Maertens, Johan A.
Cunha, Cristina
Carvalho, Agostinho
Palavras-chaveInvasive pulmonary aspergillosis
Biomarkers
Cytokines/chemokines
Bronchoalveolar lavage
Fungal diagnostics
Data29-Nov-2017
EditoraFrontiers Media
RevistaFrontiers in Microbiology
CitaçãoGonçalves, S. M., Lagrou, K., Rodrigues, C. S., Campos, C. F., Bernal-Martínez, L., Rodrigues, F., ... & Carvalho, A. (2017). Evaluation of bronchoalveolar lavage fluid cytokines as biomarkers for invasive pulmonary aspergillosis in at-risk patients. Frontiers in microbiology, 8, 2362.
Resumo(s)Background: Invasive pulmonary aspergillosis (IPA) is an infection that primarily affects immunocompromised hosts, including hematological patients and stem-cell transplant recipients. The diagnosis of IPA remains challenging, making desirable the availability of new specific biomarkers. High-throughput methods now allow us to interrogate the immune system for multiple markers of inflammation with enhanced resolution.Methods: To determine whether a signature of alveolar cytokines could be associated with the development of IPA and used as a diagnostic biomarker, we performed a nested case-control study involving 113 patients at-risk.Results: Among the 32 analytes tested, IL-beta, IL-6, IL-8, IL-17A, IL-23, and TNF a were significantly increased among patients with IPA, defining two clusters able to accurately differentiate cases of infection from controls. Genetic variants previously reported to confer increased risk of IPA compromised the production of specific cytokines and impaired their discriminatory potential toward infection. Collectively, our data indicated that IL-8 was the best performing cytokine, with alveolar levels >= 904 pg/mL predicting IPA with elevated sensitivity (90%), specificity (73%), and negative predictive value (88%).Conclusions: These findings highlight the existence of a specific profile of alveolar cytokines, with IL-8 being the dominant discriminator, whichmight be useful in supporting current diagnostic approaches for IPA.
TipoArtigo
URIhttps://hdl.handle.net/1822/50276
DOI10.3389/fmicb.2017.02362
ISSN1664-302X
Versão da editorahttps://www.frontiersin.org/articles/10.3389/fmicb.2017.02362/full
Arbitragem científicayes
AcessoAcesso aberto
Aparece nas coleções:ICVS - Artigos em revistas internacionais / Papers in international journals


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