Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/48470
Título: | Local and systemic immunomodulatory mechanisms triggered by Human Papillomavirus transformed cells: a potential role for G-CSF and neutrophils |
Autor(es): | Alvarez, Karla Lucia Fernandez Beldi, Mariana Carmezim Sarmanho, Fabiane Rossetti, Renata Ariza Marques Silveira, Caio Raony Farina Mota, Giana Rabello Andreoli, Maria Antonieta Caruso, Eliana Dias de Carvalho Kamillos, Marcia Ferreira Souza, Ana Marta Mastrocalla, Haydee Clavijo-Salomon, Maria Alejandra Barbuto, José Alexandre Marzagão Lorenzi, Noely Paula Longatto Filho, Adhemar Baracat, Edmund Chada Lopez, Rossana Verónica Mendoza Villa, Luisa Lina Tacla, Maricy Lepique, Ana Paula |
Data: | Ago-2017 |
Editora: | Nature Publishing Group |
Revista: | Scientific Reports |
Resumo(s): | Cervical cancer is the last stage of a series of molecular and cellular alterations initiated with Human Papillomavirus (HPV) infection. The process involves immune responses and evasion mechanisms, which culminates with tolerance toward tumor antigens. Our objective was to understand local and systemic changes in the interactions between HPV associated cervical lesions and the immune system as lesions progress to cancer. Locally, we observed higher cervical leukocyte infiltrate, reflected by the increase in the frequency of T lymphocytes, neutrophils and M2 macrophages, in cancer patients. We observed a strong negative correlation between the frequency of neutrophils and T cells in precursor and cancer samples, but not cervicitis. In 3D tumor cell cultures, neutrophils inhibited T cell activity, displayed longer viability and longer CD16 expression half-life than neat neutrophil cultures. Systemically, we observed higher plasma G-CSF concentration, higher frequency of immature low density neutrophils, and tolerogenic monocyte derived dendritic cells, MoDCs, also in cancer patients. Interestingly, there was a negative correlation between T cell activation by MoDCs and G-CSF concentration in the plasma. Our results indicate that neutrophils and G-CSF may be part of the immune escape mechanisms triggered by cervical cancer cells, locally and systemically, respectively. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/48470 |
DOI: | 10.1038/s41598-017-09079-3 |
ISSN: | 2045-2322 |
Versão da editora: | https://www.nature.com/articles/s41598-017-09079-3 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | ICVS - Artigos em revistas internacionais / Papers in international journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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s41598-017-09079-3.pdf | 2,65 MB | Adobe PDF | Ver/Abrir |