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https://hdl.handle.net/1822/45061
Título: | Tapirira guianensis Aubl. Extracts inhibits proliferation and migration of oral cancer cells lines |
Autor(es): | Oliveira, Renato José Silva Lopes, Gabriela Francine Camargos, Luiz Fernando Ribeiro , Ana Maciel Santos, Fábio Vieira dos Severino, Richele Priscila Severino, Vanessa Gisele Pasqualotto Terezan, Ana Paula Thomé, , Ralph Gruppi Reis, R. M. |
Palavras-chave: | HNSCC Cytotoxic activity Alkaloids Apoptosis markers |
Data: | 14-Nov-2016 |
Editora: | MDPI AG |
Revista: | International Journal of Molecular Sciences |
Citação: | Silva-Oliveira, R. J., Lopes, G. F., Camargos, L. F., Ribeiro, A. M., dos Santos, F. V., Severino, R. P., . . . Ribeiro, R. I. M. A. (2016). Tapirira guianensis Aubl. Extracts inhibit proliferation and migration of oral cancer cells lines. International Journal of Molecular Sciences, 17(11). doi: 10.3390/ijms17111839 |
Resumo(s): | Cancer of the head and neck is a group of upper aerodigestive tract neoplasms in which aggressive treatments may cause harmful side effects to the patient. In the last decade, investigations on natural compounds have been particularly successful in the field of anticancer drug research. Our aim is to evaluate the antitumor effect of Tapirira guianensis Aubl. extracts on a panel of head and neck squamous cell carcinoma (HNSCC) cell lines. Analysis of secondary metabolites classes in fractions of T. guianensis was performed using Nuclear Magnetic Resonance (NMR). Mutagenicity effect was evaluated by Ames mutagenicity assay. The cytotoxic effect, and migration and invasion inhibition were measured. Additionally, the expression level of apoptosis-related molecules (PARP, Caspases 3, and Fas) and MMP-2 was detected using Western blot. Heterogeneous cytotoxicity response was observed for all fractions, which showed migration inhibition, reduced matrix degradation, and decreased cell invasion ability. Expression levels of MMP-2 decreased in all fractions, and particularly in the hexane fraction. Furthermore, overexpression of FAS and caspase-3, and increase of cleaved PARP indicates possible apoptosis extrinsic pathway activation. Antiproliferative activity of T. guianensis extract in HNSCC cells lines suggests the possibility of developing an anticancer agent or an additive with synergic activities associated with conventional anticancer therapy. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/45061 |
DOI: | 10.3390/ijms17111839 |
ISSN: | 1661-6596 |
Versão da editora: | http://www.mdpi.com/1422-0067/17/11/1839 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | ICVS - Artigos em revistas internacionais / Papers in international journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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ijms-17-01839.pdf | 2,58 MB | Adobe PDF | Ver/Abrir |