Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/43834

TitleNeovascularization induced by the hyaluronic acid-based spongy-like hydrogels degradation products
Author(s)Silva, Lucília Pereira
Pirraco, Rogério P.
Santos, T. C.
Novoa-Carballal, R.
Cerqueira, Mariana Teixeira
Reis, R. L.
Correlo, V. M.
Marques, A. P.
KeywordsAngiogenesis
Biodegradation
gellan gum spongy-like hydrogels
Hyaluronic acid
Ischemia
Neovascularization
Issue dateNov-2016
PublisherACS Publications
JournalACS Applied Materials and Interfaces
Citationda Silva L. P., Pirraco R. P., Santos T. C., Novoa-Carballal R., Cerqueira M. T., Reis R. L., Correlo V. M., Marques A. P. Neovascularization Induced by the Hyaluronic Acid-Based Spongy- 2 Like Hydrogels Degradation Products, ACS Applied Materials and Interfaces, doi:10.1021/acsami.6b11684, 2016
Abstract(s)Neovascularization has been a major challenge in many tissue regeneration strategies. Hyaluronic acid (HA) of 3â 25 disaccharides is known to be angiogenic due to its interaction with endothelial cell receptors. This effect has been explored with HA-based structures but a transitory response is observed due to HA burst biodegradation. Herein we developed gellan gum (GG)-HA spongy-like hydrogels from semi-interpenetrating network hydrogels with different HA amounts. Enzymatic degradation was more evident in the GG-HA with high HA amount due to their lower mechanical stability, also resulting from the degradation itself, which facilitated the access of the enzyme to the HA in the bulk. GG-HA spongy-like hydrogels hyaluronidase-mediated degradation lead to the release of HA oligosaccharides of different amounts and sizes in a HA content-dependent manner which promoted in vitro proliferation of human umbilical cord vein endothelial cells (HUVECs) but not their migration. Although no effect was observed in human dermal microvascular endothelial cells (hDMECs) in vitro, the implantation of GG-HA spongy-like hydrogels in an ischemic hind limb mice model promoted neovascularization in a material-dependent manner, consistent with the in vitro degradation profile. Overall, GG-HA spongy-like hydrogels with a sustained release of HA oligomers are valuable options to improve tissue vascularization, a critical issue in several applications in the tissue engineering and regenerative medicine field.
TypeArticle
URIhttp://hdl.handle.net/1822/43834
DOI10.1021/acsami.6b11684
ISSN1944-8252
Publisher versionhttp://dx.doi.org/10.1021/acsami.6b11684
Peer-Reviewedyes
AccessRestricted access (UMinho)
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

Files in This Item:
File Description SizeFormat 
18947-Neovascularization induced by the hyaluronci acid-based spongy-like hydrogels degradation products_LdS.pdf
  Restricted access
2,22 MBAdobe PDFView/Open

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID