Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/43834

Full metadata record
DC FieldValueLanguage
dc.contributor.authorSilva, Lucília Pereirapor
dc.contributor.authorPirraco, Rogério P.por
dc.contributor.authorSantos, T. C.por
dc.contributor.authorNovoa-Carballal, R.por
dc.contributor.authorCerqueira, Mariana Teixeirapor
dc.contributor.authorReis, R. L.por
dc.contributor.authorCorrelo, V. M.por
dc.contributor.authorMarques, A. P.por
dc.date.accessioned2016-12-23T12:06:53Z-
dc.date.issued2016-11-
dc.date.submitted2016-12-
dc.identifier.citationda Silva L. P., Pirraco R. P., Santos T. C., Novoa-Carballal R., Cerqueira M. T., Reis R. L., Correlo V. M., Marques A. P. Neovascularization Induced by the Hyaluronic Acid-Based Spongy- 2 Like Hydrogels Degradation Products, ACS Applied Materials and Interfaces, doi:10.1021/acsami.6b11684, 2016por
dc.identifier.issn1944-8252por
dc.identifier.urihttp://hdl.handle.net/1822/43834-
dc.description.abstractNeovascularization has been a major challenge in many tissue regeneration strategies. Hyaluronic acid (HA) of 3â 25 disaccharides is known to be angiogenic due to its interaction with endothelial cell receptors. This effect has been explored with HA-based structures but a transitory response is observed due to HA burst biodegradation. Herein we developed gellan gum (GG)-HA spongy-like hydrogels from semi-interpenetrating network hydrogels with different HA amounts. Enzymatic degradation was more evident in the GG-HA with high HA amount due to their lower mechanical stability, also resulting from the degradation itself, which facilitated the access of the enzyme to the HA in the bulk. GG-HA spongy-like hydrogels hyaluronidase-mediated degradation lead to the release of HA oligosaccharides of different amounts and sizes in a HA content-dependent manner which promoted in vitro proliferation of human umbilical cord vein endothelial cells (HUVECs) but not their migration. Although no effect was observed in human dermal microvascular endothelial cells (hDMECs) in vitro, the implantation of GG-HA spongy-like hydrogels in an ischemic hind limb mice model promoted neovascularization in a material-dependent manner, consistent with the in vitro degradation profile. Overall, GG-HA spongy-like hydrogels with a sustained release of HA oligomers are valuable options to improve tissue vascularization, a critical issue in several applications in the tissue engineering and regenerative medicine field.por
dc.description.sponsorshipThe authors would like to acknowledge FCT for SFRH/BD/ 78025/2011 (L.d.S.), SFRH/BPD/96611/2013 (M.T.C.), SFRH/BPD/101886/2014 (R.P.P.), SFRH/BPD/101952/ 2014 (T.C.S.) grants and Xunta de Galicia for RNC fellowship and CEMUP from University of Porto for the cryoSEM analysis.por
dc.language.isoengpor
dc.publisherACS Publicationspor
dc.relationinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F78025%2F2011/PTpor
dc.relationinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F96611%2F2013/PTpor
dc.relationSFRH/BPD/101886/2014por
dc.relationSFRH/BPD/101952/ 2014por
dc.rightsrestrictedAccesspor
dc.subjectAngiogenesispor
dc.subjectBiodegradationpor
dc.subjectgellan gum spongy-like hydrogelspor
dc.subjectHyaluronic acidpor
dc.subjectIschemiapor
dc.subjectNeovascularizationpor
dc.titleNeovascularization induced by the hyaluronic acid-based spongy-like hydrogels degradation productspor
dc.typearticle-
dc.peerreviewedyespor
dc.relation.publisherversionhttp://dx.doi.org/10.1021/acsami.6b11684por
dc.commentshttp://www.3bs.uminho.pt/node/18947por
sdum.publicationstatusinfo:eu-repo/semantics/publishedVersionpor
oaire.citationStartPage33464por
oaire.citationEndPage33474por
oaire.citationIssue49por
oaire.citationTitleACS Applied Materials and Interfacespor
oaire.citationVolume8por
dc.date.updated2016-12-16T10:04:36Z-
dc.identifier.doi10.1021/acsami.6b11684por
dc.identifier.pmid27960396por
dc.subject.wosScience & Technologypor
sdum.journalACS Applied Materials and Interfacespor
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

Files in This Item:
File Description SizeFormat 
18947-Neovascularization induced by the hyaluronci acid-based spongy-like hydrogels degradation products_LdS.pdf
  Restricted access
2,22 MBAdobe PDFView/Open

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID