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TitleBiocompatibility of a self-assembled crosslinkable hyaluronic acid nanogel
Author(s)Pedrosa, Sílvia Santos
Pereira, Paula Alexandra Cunha
Correia, Alexandra
Moreira, Susana
Rocha, Huho
Gama, F. M.
Hyaluronic acid
Issue date2016
PublisherJohn Wiley and Sons
JournalMacromolecular Bioscience
CitationPedrosa, Sílvia S.; Pereira, Paula; Correia, Alexandre; Moreira, Susana; Rocha, H.; Gama, F. M., Biocompatibility of a self-assembled crosslinkable hyaluronic acid nanogel. Macromolecular Bioscience, 16(11), 1610-1620, 2016
Abstract(s)Hyaluronic acid nanogel (HyA-AT) is a redox sensitive crosslinkable nanogel, obtained through the conjugation of a thiolated hydrophobic molecule to the hyaluronic acid chain. Engineered nanogel was studied for its biocompatibility, including immunocompatibility and hemocompatability. The nanogel did not compromise the metabolic activity or cellular membrane integrity of 3T3, microvascular endothelial cells, and RAW 264.7 cell lines, as determined by the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide and lactate dehydrogenase release assays. Also, we didn't observe any apoptotic effect on these cell lines through the Annexin V-FITC test. Furthermore, the nanogel cell internalization was analyzed using murine bone marrow derived macrophages, and the in vivo and ex vivo biodistribution of the Cy5.5 labeled nanogel was monitored using a non-invasive near-infrared fluorescence imaging system. The HyA-AT nanogel exhibits fairly a long half-live in the blood stream, thus showing potential for drug delivery applications.
Publisher version
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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