Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/43001
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Campo DC | Valor | Idioma |
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dc.contributor.author | Pedrosa, Sílvia Santos | por |
dc.contributor.author | Pereira, Paula Alexandra Cunha | por |
dc.contributor.author | Correia, Alexandra | por |
dc.contributor.author | Moreira, Susana | por |
dc.contributor.author | Rocha, Huho | por |
dc.contributor.author | Gama, F. M. | por |
dc.date.accessioned | 2016-11-14T09:52:09Z | - |
dc.date.available | 2016-11-14T09:52:09Z | - |
dc.date.issued | 2016 | - |
dc.date.submitted | 2016 | - |
dc.identifier.citation | Pedrosa, Sílvia S.; Pereira, Paula; Correia, Alexandre; Moreira, Susana; Rocha, H.; Gama, F. M., Biocompatibility of a self-assembled crosslinkable hyaluronic acid nanogel. Macromolecular Bioscience, 16(11), 1610-1620, 2016 | por |
dc.identifier.issn | 1616-5187 | por |
dc.identifier.uri | https://hdl.handle.net/1822/43001 | - |
dc.description.abstract | Hyaluronic acid nanogel (HyA-AT) is a redox sensitive crosslinkable nanogel, obtained through the conjugation of a thiolated hydrophobic molecule to the hyaluronic acid chain. Engineered nanogel was studied for its biocompatibility, including immunocompatibility and hemocompatability. The nanogel did not compromise the metabolic activity or cellular membrane integrity of 3T3, microvascular endothelial cells, and RAW 264.7 cell lines, as determined by the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide and lactate dehydrogenase release assays. Also, we didn't observe any apoptotic effect on these cell lines through the Annexin V-FITC test. Furthermore, the nanogel cell internalization was analyzed using murine bone marrow derived macrophages, and the in vivo and ex vivo biodistribution of the Cy5.5 labeled nanogel was monitored using a non-invasive near-infrared fluorescence imaging system. The HyA-AT nanogel exhibits fairly a long half-live in the blood stream, thus showing potential for drug delivery applications. | por |
dc.description.sponsorship | The authors thank the FCT Strategic Project of UID/BIO/04469/2013 unit, the project RECI/ BBB-EBI/0179/2012 (FCOMP-01-0124-FEDER-027462) and the Project “BioHealth – Biotechnology and Bioengineering approaches to improve health quality”, Ref. NORTE-07-0124- FEDER-000027, co-funded by the Programa Operacional Regional do Norte (ON.2 – O Novo Norte), QREN, FEDER. The authors would like to acknowledge also the support of FCT for the PhD grant reference SFRH/BD/61516/2009. They would also like to thank Bioimaging department on Molecular Medicine Institute (IMM) in Lisbon, namely Dr José Rino and Dr António Temudo. Also thank the animal facilities in IMM (Lisbon), specially Dr. Dolores Bonaparte and Dr. Joana Marques. Finally, the authors thank Dr Africa Gonzalez and Mercedes Pelletero the performance of the studies on the activation of complement. | por |
dc.language.iso | eng | por |
dc.publisher | John Wiley and Sons | por |
dc.relation | info:eu-repo/grantAgreement/FCT/5876/147337/PT | por |
dc.relation | info:eu-repo/grantAgreement/FCT/5876-PPCDTI/126270/PT | por |
dc.relation | info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F61516%2F2009/PT | por |
dc.rights | openAccess | por |
dc.subject | Biocompatibility | por |
dc.subject | Biodistribution | por |
dc.subject | Hyaluronic acid | por |
dc.subject | Immunocompatibility | por |
dc.subject | Nanogel | por |
dc.title | Biocompatibility of a self-assembled crosslinkable hyaluronic acid nanogel | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1616-5195 | por |
dc.comments | CEB40094 | por |
sdum.publicationstatus | info:eu-repo/semantics/publishedVersion | por |
oaire.citationStartPage | 1610 | por |
oaire.citationEndPage | 1620 | por |
oaire.citationIssue | 11 | por |
oaire.citationConferencePlace | United Kingdom | - |
oaire.citationTitle | Macromolecular Bioscience | por |
oaire.citationVolume | 16 | por |
dc.date.updated | 2016-11-13T18:44:03Z | - |
dc.identifier.eissn | 1616-5195 | - |
dc.identifier.doi | 10.1002/mabi.201600221 | por |
dc.identifier.pmid | 27456215 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Macromolecular Bioscience | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_40094_1.pdf | 2,71 MB | Adobe PDF | Ver/Abrir |