Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/42384

TitleCooperation or conflict? Impact of intraspecific diversity on Escherichia coli biofilms
Author(s)Gerola, Gislaine P.
Azevedo, Andreia S.
Almeida, Carina Manuela Fernandes
Peres, Joana
Mergulhão, Filipe J.
Azevedo, Nuno F.
Issue date26-Jun-2016
CitationGerola, Gislaine P.; Azevedo, Andreia Sofia; Almeida, Carina; Peres, Joana; Mergulhão, Filipe J.; Azevedo, Nuno Filipe, Cooperation or conflict? Impact of intraspecific diversity on Escherichia coli biofilms. Biofilms 7 - Microbial Works of Art. No. P3: 13, Porto, Portugal, June 26-28, 2016. ISBN: 978-989-97478-7-6
Abstract(s)Intraspecific diversity in biofilm communities is associated with enhanced survival and growth of the individual biofilm populations. In here, we assess if this apparent cooperative behavior still holds as the number of different strains in a biofilm increases. Using E. coli as a model organism, the influence of intraspecific diversity in biofilm populations composed of up to six different E. coli strains, was assessed. Biofilm quantification was evaluated by crystal violet (CV) staining and colony forming units (CFU) counts. In general, with the increasing number of strains in a biofilm, an increase in cell counts and a decrease in matrix production was observed. This observation was confirmed by cluster analysis that indicated that after 24h of biofilm formation the best model, according to the Bayesian information criterion (BIC), consisted of three clusters that grouped together biofilms with an equal number of strains. It hence appears that increased genotypic diversity in a biofilm leads E. coli to maximize the production of its offspring, in detriment of the production of public goods (i.e. matrix components), that would be beneficial to all strains individually and the consortium as a whole. Apart from the ecological implications, these results can be explored in the area of clinical biofilms, as a decrease in matrix production might render these intraspecies biofilms more sensitive to antimicrobial agents.
TypeAbstract
URIhttp://hdl.handle.net/1822/42384
ISBN978-989-97478-7-6
Publisher versionhttps://biofilms7.com/
Peer-Reviewedyes
AccessOpen access
Appears in Collections:CEB - Resumos em Livros de Atas / Abstracts in Proceedings

Files in This Item:
File Description SizeFormat 
document_40124_1.pdf165,7 kBAdobe PDFView/Open

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID