Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/40676
Título: | Development of elastin-like recombinamer films with antimicrobial activity |
Autor(es): | da Costa, André Machado, Raul Ribeiro, Artur Collins, Tony Thiagarajan, Viruthachalam Neves-Petersen, Maria Teresa Rodríguez-Cabello, José Carlos Gomes, Andreia C Casal, Margarida |
Palavras-chave: | Animals Anti-Infective Agents Cell Line Drug Carriers Elastin Escherichia coli Humans Skin Absorption Swine Bombyx |
Data: | 9-Fev-2015 |
Editora: | American Chemical Society |
Revista: | Biomacromolecules |
Citação: | da Costa, A., Machado, R., Ribeiro, A., Collins, T., Thiagarajan, V., Neves-Petersen, M. T., . . . Casal, M. (2015). Development of Elastin-Like Recombinamer Films with Antimicrobial Activity. Biomacromolecules, 16(2), 625-635. doi: 10.1021/bm5016706 |
Resumo(s): | In the present work we explored the ABP-CM4 peptide properties from Bombyx mori for the creation of biopolymers with broad antimicrobial activity. An antimicrobial recombinant protein-based polymer (rPBP) was designed by cloning the DNA sequence coding for ABP-CM4 in frame with the N-terminus of the elastin-like recombinamer consisting of 200 repetitions of the pentamer VPAVG, here named A200. The new rPBP, named CM4-A200, was purified via a simplified nonchromatographic method, making use of the thermoresponsive behavior of the A200 polymer. ABP-CM4 peptide was also purified through the incorporation of a formic acid cleavage site between the peptide and the A200 sequence. In soluble state the antimicrobial activity of both CM4-A200 polymer and ABP-CM4 peptide was poorly effective. However, when the CM4-A200 polymer was processed into free-standing films high antimicrobial activity against Gram-positive and Gram-negative bacteria, yeasts and filamentous fungi was observed. The antimicrobial activity of CM4-A200 was dependent on the physical contact of cells with the film surface. Furthermore, CM4-A200 films did not reveal a cytotoxic effect against both normal human skin fibroblasts and human keratinocytes. Finally, we have developed an optimized ex vivo assay with pig skin demonstrating the antimicrobial properties of the CM4-A200 cast films for skin applications. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/40676 |
DOI: | 10.1021/bm5016706 |
ISSN: | 1526-4602 1525-7797 |
Arbitragem científica: | yes |
Acesso: | Acesso restrito UMinho |
Aparece nas coleções: | CBMA - Artigos/Papers DBio - Artigos/Papers |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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bm5016706.pdf Acesso restrito! | 9,02 MB | Adobe PDF | Ver/Abrir |