Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/40676
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Campo DC | Valor | Idioma |
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dc.contributor.author | da Costa, André | por |
dc.contributor.author | Machado, Raul | por |
dc.contributor.author | Ribeiro, Artur | por |
dc.contributor.author | Collins, Tony | por |
dc.contributor.author | Thiagarajan, Viruthachalam | por |
dc.contributor.author | Neves-Petersen, Maria Teresa | por |
dc.contributor.author | Rodríguez-Cabello, José Carlos | por |
dc.contributor.author | Gomes, Andreia C | por |
dc.contributor.author | Casal, Margarida | por |
dc.date.accessioned | 2016-03-07T09:55:17Z | - |
dc.date.issued | 2015-02-09 | - |
dc.identifier.citation | da Costa, A., Machado, R., Ribeiro, A., Collins, T., Thiagarajan, V., Neves-Petersen, M. T., . . . Casal, M. (2015). Development of Elastin-Like Recombinamer Films with Antimicrobial Activity. Biomacromolecules, 16(2), 625-635. doi: 10.1021/bm5016706 | por |
dc.identifier.issn | 1526-4602 | por |
dc.identifier.issn | 1525-7797 | por |
dc.identifier.uri | https://hdl.handle.net/1822/40676 | - |
dc.description.abstract | In the present work we explored the ABP-CM4 peptide properties from Bombyx mori for the creation of biopolymers with broad antimicrobial activity. An antimicrobial recombinant protein-based polymer (rPBP) was designed by cloning the DNA sequence coding for ABP-CM4 in frame with the N-terminus of the elastin-like recombinamer consisting of 200 repetitions of the pentamer VPAVG, here named A200. The new rPBP, named CM4-A200, was purified via a simplified nonchromatographic method, making use of the thermoresponsive behavior of the A200 polymer. ABP-CM4 peptide was also purified through the incorporation of a formic acid cleavage site between the peptide and the A200 sequence. In soluble state the antimicrobial activity of both CM4-A200 polymer and ABP-CM4 peptide was poorly effective. However, when the CM4-A200 polymer was processed into free-standing films high antimicrobial activity against Gram-positive and Gram-negative bacteria, yeasts and filamentous fungi was observed. The antimicrobial activity of CM4-A200 was dependent on the physical contact of cells with the film surface. Furthermore, CM4-A200 films did not reveal a cytotoxic effect against both normal human skin fibroblasts and human keratinocytes. Finally, we have developed an optimized ex vivo assay with pig skin demonstrating the antimicrobial properties of the CM4-A200 cast films for skin applications. | por |
dc.description.sponsorship | This work was supported by FEDER through POFC - COMPETE, by Portuguese funds from FCT through Project PEst-OE/BIA/UI4050/2014, and by the Spanish Minister of Economy and Competitiveness (MAT2012-38043-C02-01) and Junta de Castilla y Leon-JCyL (VA152A12-2 and VA155A12-2), Spain. The authors also thank Matadouro - Central Carnes de Entre Douro e Minho, Lda for their availability to provide pig skin. A.C. and R.M. acknowledge FCT for SFRH/BD/75882/2011 and SFRH-BPD/86470/2012 Grants, respectively. T.C. is thankful to the FCT for its support through Programa Ciencia 2008. | por |
dc.language.iso | eng | por |
dc.publisher | American Chemical Society | por |
dc.relation | info:eu-repo/grantAgreement/FCT/5876/135919/PT | por |
dc.rights | restrictedAccess | por |
dc.subject | Animals | por |
dc.subject | Anti-Infective Agents | por |
dc.subject | Cell Line | por |
dc.subject | Drug Carriers | por |
dc.subject | Elastin | por |
dc.subject | Escherichia coli | por |
dc.subject | Humans | por |
dc.subject | Skin Absorption | por |
dc.subject | Swine | por |
dc.subject | Bombyx | por |
dc.title | Development of elastin-like recombinamer films with antimicrobial activity | por |
dc.type | article | por |
dc.peerreviewed | yes | por |
sdum.publicationstatus | published | por |
oaire.citationStartPage | 625 | por |
oaire.citationEndPage | 635 | por |
oaire.citationIssue | 2 | por |
oaire.citationTitle | Biomacromolecules | por |
oaire.citationVolume | 16 | por |
dc.identifier.doi | 10.1021/bm5016706 | por |
rcaap.embargofct | Copyright | por |
dc.identifier.pmid | 25580615 | por |
dc.subject.fos | Ciências Médicas::Biotecnologia Médica | por |
dc.subject.fos | Engenharia e Tecnologia::Outras Engenharias e Tecnologias | por |
dc.subject.fos | Ciências Naturais::Ciências Biológicas | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Biomacromolecules | por |
Aparece nas coleções: | CBMA - Artigos/Papers DBio - Artigos/Papers |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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bm5016706.pdf Acesso restrito! | 9,02 MB | Adobe PDF | Ver/Abrir |