Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/40676

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dc.contributor.authorda Costa, Andrépor
dc.contributor.authorMachado, Raulpor
dc.contributor.authorRibeiro, Arturpor
dc.contributor.authorCollins, Tonypor
dc.contributor.authorThiagarajan, Viruthachalampor
dc.contributor.authorNeves-Petersen, Maria Teresapor
dc.contributor.authorRodríguez-Cabello, José Carlospor
dc.contributor.authorGomes, Andreia C.por
dc.contributor.authorCasal, Margaridapor
dc.date.accessioned2016-03-07T09:55:17Z-
dc.date.issued2015-02-09-
dc.identifier.citationda Costa, A., Machado, R., Ribeiro, A., Collins, T., Thiagarajan, V., Neves-Petersen, M. T., . . . Casal, M. (2015). Development of Elastin-Like Recombinamer Films with Antimicrobial Activity. Biomacromolecules, 16(2), 625-635. doi: 10.1021/bm5016706por
dc.identifier.issn1526-4602por
dc.identifier.issn1525-7797por
dc.identifier.urihttp://hdl.handle.net/1822/40676-
dc.description.abstractIn the present work we explored the ABP-CM4 peptide properties from Bombyx mori for the creation of biopolymers with broad antimicrobial activity. An antimicrobial recombinant protein-based polymer (rPBP) was designed by cloning the DNA sequence coding for ABP-CM4 in frame with the N-terminus of the elastin-like recombinamer consisting of 200 repetitions of the pentamer VPAVG, here named A200. The new rPBP, named CM4-A200, was purified via a simplified nonchromatographic method, making use of the thermoresponsive behavior of the A200 polymer. ABP-CM4 peptide was also purified through the incorporation of a formic acid cleavage site between the peptide and the A200 sequence. In soluble state the antimicrobial activity of both CM4-A200 polymer and ABP-CM4 peptide was poorly effective. However, when the CM4-A200 polymer was processed into free-standing films high antimicrobial activity against Gram-positive and Gram-negative bacteria, yeasts and filamentous fungi was observed. The antimicrobial activity of CM4-A200 was dependent on the physical contact of cells with the film surface. Furthermore, CM4-A200 films did not reveal a cytotoxic effect against both normal human skin fibroblasts and human keratinocytes. Finally, we have developed an optimized ex vivo assay with pig skin demonstrating the antimicrobial properties of the CM4-A200 cast films for skin applications.por
dc.description.sponsorshipThis work was supported by FEDER through POFC - COMPETE, by Portuguese funds from FCT through Project PEst-OE/BIA/UI4050/2014, and by the Spanish Minister of Economy and Competitiveness (MAT2012-38043-C02-01) and Junta de Castilla y Leon-JCyL (VA152A12-2 and VA155A12-2), Spain. The authors also thank Matadouro - Central Carnes de Entre Douro e Minho, Lda for their availability to provide pig skin. A.C. and R.M. acknowledge FCT for SFRH/BD/75882/2011 and SFRH-BPD/86470/2012 Grants, respectively. T.C. is thankful to the FCT for its support through Programa Ciencia 2008.por
dc.language.isoengpor
dc.publisherAmerican Chemical Societypor
dc.relationinfo:eu-repo/grantAgreement/FCT/5876/135919/PTpor
dc.rightsrestrictedAccesspor
dc.subjectAnimalspor
dc.subjectAnti-Infective Agentspor
dc.subjectCell Linepor
dc.subjectDrug Carrierspor
dc.subjectElastinpor
dc.subjectEscherichia colipor
dc.subjectHumanspor
dc.subjectSkin Absorptionpor
dc.subjectSwinepor
dc.subjectBombyxpor
dc.titleDevelopment of elastin-like recombinamer films with antimicrobial activitypor
dc.typearticlepor
dc.peerreviewedyespor
sdum.publicationstatuspublishedpor
oaire.citationStartPage625por
oaire.citationEndPage635por
oaire.citationIssue2por
oaire.citationTitleBiomacromoleculespor
oaire.citationVolume16por
dc.identifier.doi10.1021/bm5016706por
rcaap.embargofctCopyrightpor
dc.identifier.pmid25580615por
dc.subject.fosCiências Médicas::Biotecnologia Médicapor
dc.subject.fosEngenharia e Tecnologia::Outras Engenharias e Tecnologiaspor
dc.subject.fosCiências Naturais::Ciências Biológicaspor
dc.subject.wosScience & Technologypor
sdum.journalBiomacromoleculespor
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