Utilize este identificador para referenciar este registo: https://hdl.handle.net/1822/40236

TítuloMicroRNA-375 plays a dual role in prostate carcinogenesis
Autor(es)Costa-Pinheiro, Pedro
Ramalho-Carvalho, João
Vieira, Filipa Quintela
Torres-Ferreira, Jorge
Oliveira, Jorge
Gonçalves, Céline S.
Costa, Bruno Marques
Henrique, Rui
Jerónimo, Carmen
Palavras-chaveProstate cancer
MicroRNAs
Epigenetics
miR-375
CCND2
DataAbr-2015
EditoraBioMed Central (BMC)
RevistaClinical Epigenetics
CitaçãoCosta-Pinheiro, P., Ramalho-Carvalho, J., et. al.(2015). MicroRNA-375 plays a dual role in prostate carcinogenesis. Clinical epigenetics, 7(1), 1.
Resumo(s)Background: Prostate cancer (PCa), a highly incident and heterogeneous malignancy, mostly affects men from developed countries. Increased knowledge of the biological mechanisms underlying PCa onset and progression are critical for improved clinical management. MicroRNAs (miRNAs) deregulation is common in human cancers, and understanding how it impacts in PCa is of major importance. MiRNAs are mostly downregulated in cancer, although some are overexpressed, playing a critical role in tumor initiation and progression. We aimed to identify miRNAs overexpressed in PCa and subsequently determine its impact in tumorigenesis. Results: MicroRNA expression profiling in primary PCa and morphological normal prostate (MNPT) tissues identified 17 miRNAs significantly overexpressed in PCa. Expression of three miRNAs, not previously associated with PCa, was subsequently assessed in large independent sets of primary tumors, in which miR-182 and miR-375 were validated, but not miR-32. Significantly higher expression levels of miR-375 were depicted in patients with higher Gleason score and more advanced pathological stage, aswellaswithregionallymph nodesmetastases. Forced expression of miR-375 in PC-3 cells, which display the lowest miR-375 levels among PCa cell lines, increased apoptosis and reduced invasion ability and cell viability. Intriguingly, in 22Rv1 cells, which displayed the highest miR-375 expression, knockdown experiments also attenuated the malignant phenotype. Gene ontology analysis implicated miR-375 in several key pathways deregulated in PCa, including cell cycle and cell differentiation. Moreover, CCND2 was identified as putative miR-375 target in PCa, confirmed by luciferase assay. Conclusions: A dual role for miR-375 in prostate cancer progression is suggested, highlighting the importance of cellular context on microRNA targeting.
TipoArtigo
URIhttps://hdl.handle.net/1822/40236
DOI10.1186/s13148-015-0076-2
ISSN1868-7083
Versão da editorahttp://clinicalepigeneticsjournal.biomedcentral.com/articles/10.1186/s13148-015-0076-2
Arbitragem científicayes
AcessoAcesso aberto
Aparece nas coleções:ICVS - Artigos em revistas internacionais / Papers in international journals

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