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|Título:||Antifungal and antiaflatoxigenic properties of Lactobacillus species|
Santiago, Ana P.
Teixeira, J. A.
|Citação:||Abrunhosa, Luís; Guimarães, A.; Santiago, A.; Venâncio, Armando; Teixeira, J. A., Antifungal and antiaflatoxigenic properties of Lactobacillus species. IFT 2015 Annual Meeting and Food Expo. Chicago, USA, July 11-14, 2015.|
|Resumo(s):||Molds play an important role in spoilage of food products. It is estimated that 5 to 10% of the world foods production is lost due to fungal contamination. Further, certain fungal species produce highly toxic metabolites designated as mycotoxins. According to FAO, up to 25% of the worlds food crops have been estimated to be significantly contaminated with mycotoxins. Among all known mycotoxins, aflatoxins are the most relevant ones because they are carcinogenic. Biopreservation, defined as the control of one organism by another, has received much attention by researchers in recent years. In this field, lactic acid bacteria (LAB) are of great interest to be used as natural biopreservatives of food and feed since they have extensive probiotic properties and have been traditionally used in fermentation processes. The aim of this work is to demonstrate the potential of Lactobacillus species to control the occurrence of aflatoxigenic fungi and aflatoxins in agricultural commodities. For that several aflatoxigenic species such as Aspergillus flavus, A. parasiticus, A. nomius, A. tamari, A. arachidicola, and A. minisclerotigenes were inoculated in triplicate in MEA supplemented with 10% of sterile supernatants of different Lactobacillus species obtained from liquid MRS cultures. Supernatants of most active strains inactivated with heat, proteases and NaOH (for pH neutralization) were also tested and compared with untreated ones. The fungal radial growth and the aflatoxin concentration produced in each plate were recorded and compared. L. casei and L. plantarum supernatants were found to be most active. Growth of Aspergillus flavus in MEA after 7 days was reduced approx. by 31% and 25%, respectively for each bacteria. Aflatoxins B1 and B2 production were inhibited approx. by 97 and 87%, respectively. The antifungal and antiaflatoxigenic activity of those strains was reverted when supernatants were treated with proteolytic enzymes or their pH adjusted to 7.|
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