Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/36476

TitleCharacterization of miRNA processing machinery in the embryonic chick lung
Author(s)Moura, Rute S.
Cunha, Patrícia Vaz
Gonçalves, Carla Silva
Pinto, Jorge Correia
KeywordsChick lung
miRNA
Drosha
Dgcr8
Exportin-5
Dicer1
Issue date23-Jul-2015
PublisherSpringer Verlag
JournalCell and tissue research
Abstract(s)Lung development is a very complex process that relies on the interaction of several signaling pathways that are controlled by precise regulatory mechanisms. Recently, microRNAs (miRNAs), small non-coding regulatory RNAs, have emerged as new players involved in gene expression regulation controlling several biological processes, such as cellular differentiation, apoptosis and organogenesis, in both developmental and disease processes. Failure to correctly express some specific miRNAs or a component of their biosynthetic machinery during embryonic development is disastrous, resulting in severe abnormalities. Several miRNAs have already been identified as modulators of lung development. Regarding the spatial distribution of the processing machinery of miRNAs, only two of its members (dicer1 and argonaute) have been characterized. The present work characterizes the expression pattern of drosha, dgcr8, exportin-5 and dicer1 in early stages of the embryonic chick lung by whole mount in situ hybridization and cross-section analysis. Overall, these genes are co-expressed in dorsal and distal mesenchyme and also in growing epithelial regions. The expression pattern of miRNA processing machinery supports the previously recognized regulatory role of this mechanism in epithelial and mesenchymal morphogenesis.
TypeArticle
URIhttp://hdl.handle.net/1822/36476
DOI10.1007/s00441-015-2240-6
ISSN1432-0878
Publisher versionThe final publication is available at link.springer.com
Peer-Reviewedyes
AccessOpen access
Appears in Collections:ICVS - Artigos em Revistas Internacionais com Referee

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