Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/35867

TitleDormancy within Staphylococcus epidermidis biofilms: an immunoproteomic characterization
Author(s)Carvalhais, Virgínia Maria Dinis
Cerca, Nuno
Vilanova, M.
Vitorino, R.
Issue date2015
CitationCarvalhais, Virginia; Cerca, Nuno; Vilanova, M.; Vitorino, R., Dormancy within Staphylococcus epidermidis biofilms: an immunoproteomic characterization. EUROBIOFILMS 2015 - 4th European Congress on Microbial Biofilms. Brno, Czech Republic, June 23-26, 20, 2015.
Abstract(s)Dormant bacteria within biofilms contribute to biofilm heterogeneity. Consequently, physiological heterogeneity of biofilms may influence host immune response and tolerance to antibiotics. Recently, we described an in vitro model to modulate dormancy in S. epidermidis biofilms. Here, we present a study based on immunoproteomics, where we compared the reactive profile of S. epidermidis biofilm proteins with prevented and induced dormancy, to human sera. A total of 19 immunoreactive proteins were identified by MALDI-TOF/TOF. Most of these proteins present molecular functions, such as catalytic activity and ion binding. CodY and GpmA proteins were more reactive to sera when biofilm dormancy was induced, while FtnA and ClpP were more reactive when dormancy was prevented. This is the first work identifying protein immunoreactivity differences between bacterial biofilms with induced or prevented dormancy. Considering the importance of dormancy within biofilms, further studies on these proteins may provide insights into the mechanisms related to dormancy and help improving current understanding on how dormancy affects the host immune response.
TypeAbstract
URIhttp://hdl.handle.net/1822/35867
Peer-Reviewedyes
AccessOpen access
Appears in Collections:CEB - Resumos em Livros de Atas / Abstracts in Proceedings

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