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|Title:||Transformation of Escherichia coli JM109 using pUC19 by the Yoshida effect|
Vieira, Pedro S.
|Journal:||Journal of Microbiological Methods|
|Citation:||Mendes, Gabriel Pinto; Vieira, Pedro S.; Lanceros-Mendez, S.; Kluskens, Leon; Mota, M., Transformation of Escherichia coli JM109 using pUC19 by the Yoshida effect. Journal of Microbiological Methods, 115, 1-5, 2015|
|Abstract(s):||Transformation of non-competent Escherichia coli JM109 was accomplished using pUC19 as donor plasmid and sepiolite as the acicular material to promote cell piercing via application of friction with a polystyrene stick or a magnetic bar on the surface of a hydrogel containing agar. An automatic spreading setup was built with a conventional stirring plate and compared to manual spreading. Several parameters were optimized, namely, the agar content of the hydrogel (2%), concentration of cells (OD=1.3 corresponding to 1.4x109 bacterial cells/mL), concentration of sepiolite (0.01%), manual versus mechanical spreading (automatic spreading more consistent) and spreading time (30 sec). Efficiency values up to 4.1 x 104 CFU/µg pUC19 were obtained. The method proved to be suitable for a rapid and low cost transformation of non-competent E. coli JM109, where higher values of efficiency do not need to be attained.|
|Appears in Collections:||CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series|